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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,9,22]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Malaria remains a major public health issue worldwide and world health organization estimates \u223c198 million cases and \u223c584,000 deaths in the year of 2013 alone due to malaria. Lack of an effective vaccine and rapid emergence of drug resistance are two major causes of this persistent problem. Understanding the biology of the parasite and studies of its gene function are essential to identify potential drug targets. Here we report a morpholino oligomer (MO)-based approach to alter gene expression via inhibition of post-transcriptional processes or by targeting mRNAs for degradation. The ease in design of the MO molecules presents a possibility for their use in large-scale genome functional analyses and possibly in malaria therapy.<\/jats:p>","DOI":"10.1073\/pnas.1515864112","type":"journal-article","created":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T22:21:21Z","timestamp":1441750881000},"page":"11935-11940","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in\n                    <i>Plasmodium falciparum<\/i>"],"prefix":"10.1073","volume":"112","author":[{"given":"Aprajita","family":"Garg","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Wesolowski","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulce","family":"Alonso","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk W.","family":"Deitsch","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choukri","family":"Ben Mamoun","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidney","family":"Altman","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,9,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp239"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3063"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086802"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203516109"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2143"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-83"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6329"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073783"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","unstructured":"J Summerton Morpholino antisense oligomers: The case for an RNase H-independent structural type. 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