{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:18:14Z","timestamp":1791393494457,"version":"4.3.3"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"40","license":[{"start":{"date-parts":[[2016,3,21]],"date-time":"2016-03-21T00:00:00Z","timestamp":1458518400000},"content-version":"vor","delay-in-days":182,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001826","name":"Netherlands Organisation for Health Research and Development","doi-asserted-by":"publisher","award":["VIDI 016.146.353"],"award-info":[{"award-number":["VIDI 016.146.353"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"Netherlands Organisation for Health Research and Development","doi-asserted-by":"publisher","award":["TAS 40-41400-98-1108"],"award-info":[{"award-number":["TAS 40-41400-98-1108"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["MCBO PhD program"],"award-info":[{"award-number":["MCBO PhD program"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,10,6]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Microvillus inclusion disease (MVID) is a rare intestinal enteropathy resulting in severe diarrhoea in neonates. Here, we have generated an intestine-specific knockout mouse model for Myosin Vb, the gene causing MVID in the majority of human patients. Our mouse model completely recapitulates the intestinal human MVID phenotype, including severe diarrhoea, loss of microvilli, occurrence of microvillus inclusions, and subapical secretory granules in villus enterocytes. In addition, we identify a newly identified role of Myo5b in trafficking of basolateral proteins, in the apical localization of the brush border membrane fusion protein syntaxin 3 (STX3), and in early differentiation of enterocytes. Our data indicate a role of MYO5B in regulating polarity of epithelial cells and have important implications for future treatment options for MVID patients.<\/jats:p>","DOI":"10.1073\/pnas.1516672112","type":"journal-article","created":{"date-parts":[[2015,9,21]],"date-time":"2015-09-21T23:08:42Z","timestamp":1442876922000},"page":"12408-12413","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":89,"title":["An inducible mouse model for microvillus inclusion disease reveals a role for myosin Vb in apical and basolateral trafficking"],"prefix":"10.1073","volume":"112","author":[{"given":"Kerstin","family":"Schneeberger","sequence":"first","affiliation":[{"name":"Division of Paediatrics, Department of Paediatric Gastroenterology, Wilhelmina Children\u2019s Hospital, University Medical Centre (UMC) Utrecht, 3584 EA, Utrecht, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg F.","family":"Vogel","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, Biocenter, Medical University Innsbruck, A-6020 Innsbruck, Austria;"},{"name":"Division of Histology and Embryology, Medical University Innsbruck, A-6020 Innsbruck Austria;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Teunissen","sequence":"additional","affiliation":[{"name":"Division of Paediatrics, Department of Paediatric Gastroenterology, Wilhelmina Children\u2019s Hospital, University Medical Centre (UMC) Utrecht, 3584 EA, Utrecht, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenique D.","family":"van Ommen","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Immunology and Department of Paediatric Pulmonology, Wilhelmina Children\u2019s Hospital, UMC Utrecht, 3584 EA, Utrecht, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Begthel","sequence":"additional","affiliation":[{"name":"Hubrecht Institute for Developmental Biology and Stem Cell Research, Royal Dutch Academy of Sciences and UMC Utrecht, 3584 CT, Utrecht, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Layla","family":"El Bouazzaoui","sequence":"additional","affiliation":[{"name":"Division of Paediatrics, Department of Paediatric Gastroenterology, Wilhelmina Children\u2019s Hospital, University Medical Centre (UMC) Utrecht, 3584 EA, Utrecht, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke H. 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