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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,4,26]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Animal toxin peptides typically exhibit high affinity and selectivity for specific subclasses of cell receptors that account for their activity. Posttranslational modifications of toxin sequences may occur in the venom gland machinery, but there is no example so far of toxins being subjected to such modifications ex situ after delivery to target cells. Here, we provide evidence that the cell permeant maurocalcin (MCa), found in scorpion venom, undergoes PKA-mediated phosphorylation in cellulo, and that posttranslational modification leads to its complete pharmacological reprogramming activity toward ryanodine receptor type 1 (RyR1), a key player in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis in excitable cells. Phosphorylation converts MCa from a potent stabilizer of RyR1 channel substates to a negative allosteric modulator that mitigates RyR1 channel Ca\n                    <jats:sup>2+<\/jats:sup>\n                    leak.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1517342113","type":"journal-article","created":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T00:43:10Z","timestamp":1460421790000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["In cellulo phosphorylation induces pharmacological reprogramming of maurocalcin, a cell-penetrating venom peptide"],"prefix":"10.1073","volume":"113","author":[{"given":"Michel","family":"Ronjat","sequence":"first","affiliation":[{"name":"INSERM U836, LabEx Ion Channel, Science and Therapeutics, Grenoble Institute of Neuroscience, 38042 Grenoble, France;"},{"name":"Universit\u00e9 Grenoble Alpes, 38042 Grenoble, 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France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Chahboun","sequence":"additional","affiliation":[{"name":"INSERM U836, LabEx Ion Channel, Science and Therapeutics, Grenoble Institute of Neuroscience, 38042 Grenoble, France;"},{"name":"Universit\u00e9 Grenoble Alpes, 38042 Grenoble, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Lesage","sequence":"additional","affiliation":[{"name":"CNRS UMR 7275, LabEx Ion Channel, Science and Therapeutics, Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, F-06560 Valbonne, France;"},{"name":"Universit\u00e9 de Nice Sophia Antipolis, F-06560 Nice, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Darbon","sequence":"additional","affiliation":[{"name":"Architecture et Fonction des Macromol\u00e9cules Biologiques-CNRS UMR 7257, Aix-Marseille Universit\u00e9, 13009 Marseille, 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