{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:22:56Z","timestamp":1790346176640,"version":"4.1.0"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"46","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM29513"],"award-info":[{"award-number":["R01 GM29513"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,11,17]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Centrosomes organize the microtubule cytoskeleton in interphase and mitosis. During mitosis, the centrosomes are important for the formation and positioning of the bipolar mitotic spindle on which chromosomes are segregated. The presence of more than two centrosomes can drive mitotic chromosome segregation errors and the formation of aneuploid cells. Centrosome amplification is a common feature of aneuploid cancer cells, but a long-standing question is whether this is a cause or a consequence of tumor development. To assess this question, we generated mice in which centrosome amplification can be induced widely. Despite chronic centrosome amplification, tumorigenesis was not enhanced, demonstrating that an excess of centrosomes is not sufficient to drive tumor development.<\/jats:p>","DOI":"10.1073\/pnas.1519388112","type":"journal-article","created":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T20:40:47Z","timestamp":1446496847000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":84,"title":["Chronic centrosome amplification without tumorigenesis"],"prefix":"10.1073","volume":"112","author":[{"given":"Benjamin","family":"Vitre","sequence":"first","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Holland","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Kulukian","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ofer","family":"Shoshani","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maretoshi","family":"Hirai","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy, University of California at San Diego, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Maldonado","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Cho","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihane","family":"Boubaker","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah A.","family":"Swing","sequence":"additional","affiliation":[{"name":"Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD 21702;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lino","family":"Tessarollo","sequence":"additional","affiliation":[{"name":"Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD 21702;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia M.","family":"Evans","sequence":"additional","affiliation":[{"name":"Skaggs School of Pharmacy, University of California at San Diego, La Jolla, CA 92093;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don W.","family":"Cleveland","sequence":"additional","affiliation":[{"name":"San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA 92093;"},{"name":"Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"e_1_3_3_1_2","volume-title":"Zellen-Studien: \u00dcber die Natur der Centrosomen","author":"Boveri T","year":"1900","unstructured":"T Boveri Zellen-Studien: \u00dcber die Natur der Centrosomen (Gustav Fischer, Jena, Germany, 1900)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2008.06.004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.10.036"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2718"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.10.006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08136"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006564"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13277"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032479999"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403779"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.7.1122"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.01.066"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.07.029"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.039"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.207027.112"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2746"},{"key":"e_1_3_3_17_2","unstructured":"T Boveri Zur Frage der Entstehung M aligner Tumoren (Gustav Fischer Jena Germany). (1914)."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00338-5"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1320"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.11.042"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.04.036"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00313-X"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<113::AID-GENE3>3.0.CO;2-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0597"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1518376112"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3524"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1744"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.7.882"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/22.1.99"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng747"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.120"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.15.8551"},{"key":"e_1_3_3_34_2","volume-title":"Concerning the Origin of Malignant Tumours","author":"Boveri T","year":"2008","unstructured":"T Boveri Concerning the Origin of Malignant Tumours (Cold Spring Harbor Lab Press, Cold Spring Harbor, NY, 2008)."},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(00)00384-8"},{"key":"e_1_3_3_36_2","first-page":"1398","article-title":"Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas","volume":"63","author":"Pihan GA","year":"2003","unstructured":"GA Pihan, J Wallace, Y Zhou, SJ Doxsey, Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas. Cancer Res 63, 1398\u20131404 (2003).","journal-title":"Cancer Res"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1735"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-12-1043"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa5111"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201502089"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09414"},{"key":"e_1_3_3_42_2","unstructured":"F Mitelman B Johansson F Mertens Mitelman Database of Chromosome Aberrations and Gene Fusions in Cancer. Available at cgap.nci.nih.gov\/Chromosomes\/Mitelman. Accessed May 1 2012. (2012)."},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-092910-154234"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.031"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04217"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.633891"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020242"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_3_3_49_2","doi-asserted-by":"crossref","unstructured":"DA Conner Mouse embryo fibroblast (MEF) feeder cell preparation. Curr Protoc Mol Biol Chap. 23 Unit 23.2. (2001).","DOI":"10.1002\/0471142727.mb2302s51"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.9230"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1519388112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1519388112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T01:43:30Z","timestamp":1649814210000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1519388112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,2]]},"references-count":50,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2015,11,17]]}},"alternative-id":["10.1073\/pnas.1519388112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1519388112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,2]]},"assertion":[{"value":"2015-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}