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However, the ACP recognition mechanism has remained elusive because the structural determination of an AT\u2013ACP complex is hampered by the weak and transient interactions between them. Herein, we describe the first crystal structure of the AT\u2013ACP complex. To stabilize the weak protein\u2013protein interaction sufficiently for analysis, we prepared a covalent AT\u2013ACP complex using a cross-linking reagent for crystallization. The determined AT\u2013ACP complex structure provides detailed mechanistic insights into ACP recognition by AT.<\/jats:p>","DOI":"10.1073\/pnas.1520042113","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T23:45:31Z","timestamp":1454370331000},"page":"1802-1807","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":90,"title":["Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis"],"prefix":"10.1073","volume":"113","author":[{"given":"Akimasa","family":"Miyanaga","sequence":"first","affiliation":[{"name":"Department of Chemistry, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Iwasawa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumitaka","family":"Kudo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Eguchi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,2,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1002\/anie.200806121","article-title":"The biosynthetic logic of polyketide diversity","volume":"48","author":"Hertweck C","year":"2009","unstructured":"C Hertweck, The biosynthetic logic of polyketide diversity. 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