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U.S.A."],"published-print":{"date-parts":[[2016,5,24]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Gain-of-function screens using overexpression genomic libraries are powerful tools for discovering drug target\/resistance genes, but several limitations make this technique less amenable to high-throughput screening. Using cosmid-based functional screening coupled to next-generation sequencing, an approach that we term Cosmid Sequencing (or \u201cCos-Seq\u201d), we followed the dynamics of cosmid enrichment during drug pressure in\n                    <jats:italic>Leishmania<\/jats:italic>\n                    , the parasite responsible for leishmaniasis, a neglected tropical disease. This improved and sensitive method has led to the identification and functional characterization of an unprecedented number of drug target\/resistance genes against all drugs currently used to treat leishmaniasis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1520693113","type":"journal-article","created":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T00:09:33Z","timestamp":1462838973000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":85,"title":["Cos-Seq for high-throughput identification of drug target and resistance mechanisms in the protozoan parasite\n                    <i>Leishmania<\/i>"],"prefix":"10.1073","volume":"113","author":[{"given":"\u00c9lodie","family":"Gazanion","sequence":"first","affiliation":[{"name":"Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Qu\u00e9bec, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 4G2;"},{"name":"D\u00e9partement de Microbiologie, Infectiologie, et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4834-4956","authenticated-orcid":false,"given":"Christopher","family":"Fern\u00e1ndez-Prada","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Qu\u00e9bec, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 4G2;"},{"name":"D\u00e9partement de Microbiologie, Infectiologie, et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Papadopoulou","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Qu\u00e9bec, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 4G2;"},{"name":"D\u00e9partement de Microbiologie, Infectiologie, et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Leprohon","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Qu\u00e9bec, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 4G2;"},{"name":"D\u00e9partement de Microbiologie, Infectiologie, et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Ouellette","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Qu\u00e9bec, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 4G2;"},{"name":"D\u00e9partement de Microbiologie, Infectiologie, et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, Qu\u00e9bec, QC, Canada G1V 0A6"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,5,9]]},"reference":[{"key":"e_1_3_4_1_2","volume-title":"Sustaining the Drive to Overcome the Global Impact of Neglected Tropical Diseases: Second WHO Report on Neglected Tropical Diseases","author":"World, Health Organization","year":"2013","unstructured":"; World, Health Organization Sustaining the Drive to Overcome the Global Impact of Neglected Tropical Diseases: Second WHO Report on Neglected Tropical Diseases (World Health Organization, Geneva, Switzerland, 2013)."},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/CMR.19.1.111-126.2006","article-title":"Drug resistance in leishmaniasis","volume":"19","author":"Croft SL","year":"2006","unstructured":"SL Croft, S Sundar, AH Fairlamb, Drug resistance in leishmaniasis. 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