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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,2,16]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    is the causative agent of Legionnaires\u2019 disease. It translocates a large repertoire of effectors into the host cell through a specialized secretion system to subvert cellular defenses. A key characteristic of this pathogen is that the majority of its effectors are encoded by eukaryotic-like genes acquired through horizontal gene transfer. We determined the crystal structure of one of these effectors, sphingosine-1 phosphate lyase (\n                    <jats:italic>Lp<\/jats:italic>\n                    Spl), and show that it has high similarity with its eukaryotic homologue. We demonstrate that\n                    <jats:italic>Lp<\/jats:italic>\n                    Spl possesses lyase activity and that it disrupts sphingolipid metabolism in the host cells.\n                    <jats:italic>Lp<\/jats:italic>\n                    Spl plays a critical and previously unknown role in decreasing autophagy and is a unique virulence factor facilitating intracellular replication of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.1522067113","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T23:45:31Z","timestamp":1454370331000},"page":"1901-1906","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":144,"title":["<i>Legionella pneumophila<\/i>\n                    S1P-lyase targets host sphingolipid metabolism and restrains autophagy"],"prefix":"10.1073","volume":"113","author":[{"given":"Monica","family":"Rolando","sequence":"first","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Escoll","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Nora","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00eblle","family":"Botti","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants-Malades, INSERM U1151-CNRS UMR 8253, 75993 Paris, France;"},{"name":"Universit\u00e9 Paris Descartes-Sorbonne Paris Cit\u00e9, 75993 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Boitez","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants-Malades, INSERM U1151-CNRS UMR 8253, 75993 Paris, France;"},{"name":"Universit\u00e9 Paris Descartes-Sorbonne Paris Cit\u00e9, 75993 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4584-5843","authenticated-orcid":false,"given":"Carmen","family":"Bedia","sequence":"additional","affiliation":[{"name":"INSERM UMR1037, Centre de Recherches en Canc\u00e9rologie de Toulouse, 31432 Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Daniels","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada M5S 3E5;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilu","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and the Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Stogios","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada M5S 3E5;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Skarina","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada M5S 3E5;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Christophe","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Dervins-Ravault","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Cazalet","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biologie des Bact\u00e9ries Intracellulaires, 75724 Paris, France;"},{"name":"CNRS UMR 3525, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Z\u00fcrich, 8006, Zurich, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thusitha W. 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