{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T11:42:49Z","timestamp":1791027769951,"version":"4.1.0"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,7,23]]},"abstract":"<jats:p>\n                    The PilB protein of\n                    <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    has been reported to be involved in the regulation of pilin gene transcription, but it also possesses significant homology to the peptide methionine sulfoxide reductase family of enzymes, specifically MsrA and MsrB from\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . MsrA and MsrB in\n                    <jats:italic>E. coli<\/jats:italic>\n                    are able to reduce methionine sulfoxide residues in proteins to methionines. In addition, the gonococcal PilB protein encodes for both MsrA and MsrB activity associated with the repair of oxidative damage to proteins. In this work, we demonstrate that the PilB protein of\n                    <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    is not involved in pilus expression. Additionally, we show that wild-type\n                    <jats:italic>N. gonorrhoeae<\/jats:italic>\n                    produces two forms of this polypeptide, one of which contains a signal sequence and is secreted from the bacterial cytoplasm to the outer membrane; the other lacks a signal sequence and is cytoplasmic. Furthermore, we show that the secreted form of the PilB protein is involved in survival in the presence of oxidative damage.\n                  <\/jats:p>","DOI":"10.1073\/pnas.152334799","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T18:26:28Z","timestamp":1027880788000},"page":"10108-10113","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":120,"title":["The outer membrane localization of the\n                    <i>Neisseria gonorrhoeae<\/i>\n                    MsrA\/B is involved in survival against reactive oxygen species"],"prefix":"10.1073","volume":"99","author":[{"given":"Eric P.","family":"Skaar","sequence":"first","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah M.","family":"Tobiason","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Quick","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph C.","family":"Judd","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Weissbach","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frantzy","family":"Etienne","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Brot","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Steven","family":"Seifert","sequence":"additional","affiliation":[{"name":"Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Searle 6-497, Chicago, IL 60611; Division of Biological Sciences, University of Montana, 32 Campus Drive 4824 (HS104), Missoula, MT 59812; Center for Molecular Biology and Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431; Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021; and Department of Microbiology and Immunology, Weill Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,7,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006565-199408000-00014"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115770"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.43.100189.001005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.Suppl.S83"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.16.8841"},{"key":"e_1_3_3_6_2","first-page":"103","volume":"13","author":"Spitznagel J K","year":"1977","unstructured":"J K Spitznagel Prog Clin Biol Res 13, 103\u2013131 (1977).","journal-title":"Prog Clin Biol Res"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/10.Supplement_2.S450"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00231175"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.2.631-641.1986"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1232-1238.1993"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02460.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.85.6.1274-1279.1963"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.137.3.571"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03335.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.44.26045"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.3.731-739.1999"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7985"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6463"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105509200"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.19.10268"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90592-1"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.4.2155"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7483"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00888-1"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.19.14167"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(94)00158-G"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00908-9"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77830-0"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9932"},{"key":"e_1_3_3_30_2","first-page":"348","volume":"9","author":"Lowther W T","year":"2002","unstructured":"W T Lowther, H Weissbach, F Etienne, N Brot, B W Matthews Nat Struc Biol 9, 348\u2013352 (2002).","journal-title":"Nat Struc Biol"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112350200"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00810-4"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02463.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1600-1609.2001"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.6.1782"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00935.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.14.1.266-270.1976"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90283-4"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.213-217.1998"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(74)31070-1"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-142-9-2481"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.Suppl.S41"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0206"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(88)90194-7"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(75)90350-2"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00890-5"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.8129"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01834.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.3.502-507.1995"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161295398"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9585"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15036"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(96)00006-7"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.9.2864-2871.1991"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90143-6"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.152334799","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T01:32:53Z","timestamp":1649813573000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.152334799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7,2]]},"references-count":55,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2002,7,23]]}},"alternative-id":["10.1073\/pnas.152334799"],"URL":"https:\/\/doi.org\/10.1073\/pnas.152334799","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7,2]]},"assertion":[{"value":"2002-06-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}