{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:35:22Z","timestamp":1792456522645,"version":"4.5.0"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"19","license":[{"start":{"date-parts":[[2016,10,26]],"date-time":"2016-10-26T00:00:00Z","timestamp":1477440000000},"content-version":"vor","delay-in-days":183,"URL":"http:\/\/www.pnas.org\/preview_site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1332349"],"award-info":[{"award-number":["1332349"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,5,10]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Biomineralization is a promising, yet complex, route toward the scalable and green biomanufacturing of functional nanomaterials, involving multiple biomolecules acting in unison to control mineralization and crystal structure. Unraveling the interdependent complexity of biomineralization is a barrier to completely realize this approach. We distill this complexity to a single enzyme that both catalyzes the formation of the reactive precursors required for mineralization and templates nanocrystal growth in solution. This is the first report of a single enzyme capable of providing all of the required functionality for active biomineralization from otherwise unreactive solution. This work provides insight into the mechanism of metal sulfide biomineralization and is an example of the elegance in green functional material synthesis achievable through engineered biomineralization.<\/jats:p>","DOI":"10.1073\/pnas.1523633113","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T00:06:15Z","timestamp":1461715575000},"page":"5275-5280","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":108,"title":["Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties"],"prefix":"10.1073","volume":"113","author":[{"given":"Robert","family":"Dunleavy","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Kiely","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015;"},{"name":"Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"McIntosh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan W.","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015;"},{"name":"Program in Bioengineering, Lehigh University, Bethlehem, PA 18015"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,4,26]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780198508823.001.0001","volume-title":"Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry","author":"Mann S","year":"2001","unstructured":"S Mann Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry (Oxford Univ Press, New York, 2001)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr8002328"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201106715"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.260496497"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5442.1129"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200500616"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6234"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10402"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5245.67"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200300381"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm9503285"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/381056a0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200600235"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04382"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr078258w"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2007.10.007"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2003.01263.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1039\/B410283E"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1039\/b510113a"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116958109"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200600163"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(97)10006-X"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.3485295"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832310100"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/B307593A"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep03832"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm0628799"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1039\/b920688d"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705806"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200500026"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2007.045"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5GC00194C"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100660"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1134\/S0020168508100038"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfa.2004.11.024"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie060963s"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.08.022"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-161"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2008.12.025"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.07.017"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001539"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.1.7-14.1993"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)70477-8"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805459200"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2009.00546.x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm034081k"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5251.933"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.45.3410"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/19\/43\/435708"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43492-3"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/bio.981"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2005.04.036"},{"key":"e_1_3_3_53_2","doi-asserted-by":"crossref","unstructured":"KJ Petty Metal-chelate affinity chromatography. Curr Protoc Protein Sci 4(9.4):9.4.1\u20139.4.16. (2001).","DOI":"10.1002\/0471140864.ps0904s04"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808026200"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1523633113","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1523633113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T20:22:40Z","timestamp":1718482960000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1523633113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,26]]},"references-count":54,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2016,5,10]]}},"alternative-id":["10.1073\/pnas.1523633113"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1523633113","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,26]]},"assertion":[{"value":"2016-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}