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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,9,20]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Membrane-deforming proteins cooperate with the cytoskeleton to sculpt lipid bilayers into complex and dynamic geometries, but we still do not understand how their activities are temporally and spatially regulated in cells. Here we show that the neuronal membrane remodeling protein Nervous Wreck (Nwk) is autoinhibited by intramolecular interactions between its membrane binding F-BAR domain and its C-terminal SRC homology 3 (SH3) domains. These autoinhibitory interactions control Fes\/Cip4 homology-Bin\/Amphiphysin\/Rvs167 (F-BAR)\u2013mediated membrane remodeling and also, unexpectedly, inhibit SH3-mediated actin cytoskeleton assembly. Uncoupling these dual autoregulatory mechanisms in the fruit fly leads to excess neuronal synapse growth. Thus, coordinated autoregulation couples membrane remodeling and SH3 domain activities, and is critical for proper control of neuronal shape and size.<\/jats:p>","DOI":"10.1073\/pnas.1524412113","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T21:55:26Z","timestamp":1473198926000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck"],"prefix":"10.1073","volume":"113","author":[{"given":"Tatiana B.","family":"Stanishneva-Konovalova","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte F.","family":"Kelley","sequence":"additional","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania L.","family":"Eskin","sequence":"additional","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily M.","family":"Messelaar","sequence":"additional","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Wasserman","sequence":"additional","affiliation":[{"name":"Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga S.","family":"Sokolova","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Faculty of Biology, M.V. 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