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The antibodies, cell isolation methods, and RNAseq profiles presented here will greatly facilitate our understanding of microglial function in health and disease.<\/jats:p>","DOI":"10.1073\/pnas.1525528113","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T23:10:11Z","timestamp":1455664211000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1724,"title":["New tools for studying microglia in the mouse and human CNS"],"prefix":"10.1073","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1642-2887","authenticated-orcid":false,"given":"Mariko L.","family":"Bennett","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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A possible role for macrophages in regeneration. J Exp Med 165, 1218\u20131223 (1987).","journal-title":"J Exp Med"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1002\/(SICI)1098-1136(199603)16:3<227::AID-GLIA5>3.0.CO;2-Z","article-title":"Cellular reactions at the lesion site after crushing of the rat optic nerve","volume":"16","author":"Frank M","year":"1996","unstructured":"M Frank, H Wolburg, Cellular reactions at the lesion site after crushing of the rat optic nerve. Glia 16, 227\u2013240 (1996).","journal-title":"Glia"},{"key":"e_1_3_4_28_2","first-page":"2348","article-title":"Isolation of brain and spinal cord mononuclear cells using percoll gradients","author":"Pino PA","year":"2011","unstructured":"PA Pino, AE Cardona, Isolation of brain and spinal cord mononuclear cells using percoll gradients. J Vis Exp, pp. 2348 (2011).","journal-title":"J Vis Exp"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1186\/1742-2094-9-147","article-title":"Efficient isolation of live microglia with preserved phenotypes from adult mouse brain","volume":"9","author":"Nikodemova M","year":"2012","unstructured":"M Nikodemova, JJ Watters, Efficient isolation of live microglia with preserved phenotypes from adult mouse brain. J Neuroinflammation 9, 147 (2012).","journal-title":"J Neuroinflammation"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"11929","DOI":"10.1523\/JNEUROSCI.1860-14.2014","article-title":"An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex","volume":"34","author":"Zhang Y","year":"2014","unstructured":"Y Zhang, , An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. 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Immunity 43, 92\u2013106 (2015).","journal-title":"Immunity"},{"key":"e_1_3_4_35_2","unstructured":"R Suzuki H Shimodaira pvclust: Hierarchial clustering with P-values via multiscale bootstrap resampling. Available at cran.r-project.org\/web\/packages\/pvclust\/index.html. Accessed May 22 2015. (2014)."},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"16064","DOI":"10.1523\/JNEUROSCI.4158-11.2011","article-title":"The role of microglia in the healthy brain","volume":"31","author":"Tremblay ME","year":"2011","unstructured":"ME Tremblay, , The role of microglia in the healthy brain. J Neurosci 31, 16064\u201316069 (2011).","journal-title":"J Neurosci"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1097\/00005072-199509000-00010","article-title":"Microglial turnover in the injured CNS: Activated microglia undergo delayed DNA fragmentation following peripheral nerve injury","volume":"54","author":"Gehrmann J","year":"1995","unstructured":"J Gehrmann, RB Banati, Microglial turnover in the injured CNS: Activated microglia undergo delayed DNA fragmentation following peripheral nerve injury. J Neuropathol Exp Neurol 54, 680\u2013688 (1995).","journal-title":"J Neuropathol Exp Neurol"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.pain.2007.05.002","article-title":"Characterization of cell proliferation in rat spinal cord following peripheral nerve injury and the relationship with neuropathic pain","volume":"135","author":"Echeverry S","year":"2008","unstructured":"S Echeverry, XQ Shi, J Zhang, Characterization of cell proliferation in rat spinal cord following peripheral nerve injury and the relationship with neuropathic pain. Pain 135, 37\u201347 (2008).","journal-title":"Pain"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s10571-008-9332-8","article-title":"Peripheral nerve lesion induces an up-regulation of Spy1 in rat spinal cord","volume":"29","author":"Huang Y","year":"2009","unstructured":"Y Huang, , Peripheral nerve lesion induces an up-regulation of Spy1 in rat spinal cord. Cell Mol Neurobiol 29, 403\u2013411 (2009).","journal-title":"Cell Mol Neurobiol"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nature09513","article-title":"Pericytes are required for blood-brain barrier integrity during embryogenesis","volume":"468","author":"Daneman R","year":"2010","unstructured":"R Daneman, L Zhou, AA Kebede, BA Barres, Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature 468, 562\u2013566 (2010).","journal-title":"Nature"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1126\/science.277.5323.242","article-title":"Pericyte loss and microaneurysm formation in PDGF-B-deficient mice","volume":"277","author":"Lindahl P","year":"1997","unstructured":"P Lindahl, BR Johansson, P Lev\u00e9en, C Betsholtz, Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 277, 242\u2013245 (1997).","journal-title":"Science"},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1242\/dev.126.3.457","article-title":"Defective oligodendrocyte development and severe hypomyelination in PDGF-A knockout mice","volume":"126","author":"Fruttiger M","year":"1999","unstructured":"M Fruttiger, , Defective oligodendrocyte development and severe hypomyelination in PDGF-A knockout mice. Development 126, 457\u2013467 (1999).","journal-title":"Development"},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"E440","DOI":"10.1073\/pnas.1104977108","article-title":"Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC","volume":"108","author":"Kucukdereli H","year":"2011","unstructured":"H Kucukdereli, , Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. Proc Natl Acad Sci USA 108, E440\u2013E449 (2011).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_44_2","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1056\/NEJM198310133091507","article-title":"Hypoxanthine-guanine phosphoribosyltransferase deficiency. The molecular basis of the clinical syndromes","volume":"309","author":"Wilson JM","year":"1983","unstructured":"JM Wilson, AB Young, AB Young, WN Kelley, Hypoxanthine-guanine phosphoribosyltransferase deficiency. The molecular basis of the clinical syndromes. N Engl J Med 309, 900\u2013910 (1983).","journal-title":"N Engl J Med"},{"key":"e_1_3_4_45_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.biopsych.2005.10.024","article-title":"Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond","volume":"60","author":"Tunbridge EM","year":"2006","unstructured":"EM Tunbridge, PJ Harrison, DR Weinberger, Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond. Biol Psychiatry 60, 141\u2013151 (2006).","journal-title":"Biol Psychiatry"},{"key":"e_1_3_4_46_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1056\/NEJMoa1211851","article-title":"TREM2 variants in Alzheimer\u2019s disease","volume":"368","author":"Guerreiro R","year":"2013","unstructured":"R Guerreiro, , TREM2 variants in Alzheimer\u2019s disease. N Engl J Med; Alzheimer Genetic Analysis Group 368, 117\u2013127 (2013).","journal-title":"N Engl J Med"},{"key":"e_1_3_4_47_2","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.cell.2015.01.049","article-title":"TREM2 lipid sensing sustains the microglial response in an Alzheimer\u2019s disease model","volume":"160","author":"Wang Y","year":"2015","unstructured":"Y Wang, , TREM2 lipid sensing sustains the microglial response in an Alzheimer\u2019s disease model. Cell 160, 1061\u20131071 (2015).","journal-title":"Cell"},{"key":"e_1_3_4_48_2","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.neuron.2012.03.026","article-title":"Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner","volume":"74","author":"Schafer DP","year":"2012","unstructured":"DP Schafer, , Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner. Neuron 74, 691\u2013705 (2012).","journal-title":"Neuron"},{"key":"e_1_3_4_49_2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/nature12776","article-title":"Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways","volume":"504","author":"Chung","year":"2013","unstructured":"Chung, , Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways. Nature 504, 394\u2013400 (2013).","journal-title":"Nature"},{"key":"e_1_3_4_50_2","doi-asserted-by":"crossref","first-page":"e101","DOI":"10.1182\/blood-2011-03-339705","article-title":"Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes","volume":"118","author":"Rowley JW","year":"2011","unstructured":"JW Rowley, , Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood 118, e101\u2013e111 (2011).","journal-title":"Blood"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/336487a0","article-title":"Laminin receptor on platelets is the integrin VLA-6","volume":"336","author":"Sonnenberg A","year":"1988","unstructured":"A Sonnenberg, PW Modderman, F Hogervorst, Laminin receptor on platelets is the integrin VLA-6. Nature 336, 487\u2013489 (1988).","journal-title":"Nature"},{"key":"e_1_3_4_52_2","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/0092-8674(95)90126-4","article-title":"Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration","volume":"83","author":"Langenbach R","year":"1995","unstructured":"R Langenbach, , Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell 83, 483\u2013492 (1995).","journal-title":"Cell"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1096\/fasebj.5.9.1907252","article-title":"Human platelet\/erythroleukemia cell prostaglandin G\/H synthase: cDNA cloning, expression, and gene chromosomal assignment","volume":"5","author":"Funk CD","year":"1991","unstructured":"CD Funk, LB Funk, ME Kennedy, AS Pong, GA Fitzgerald, Human platelet\/erythroleukemia cell prostaglandin G\/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5, 2304\u20132312 (1991).","journal-title":"FASEB J"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1126\/science.1110647","article-title":"Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo","volume":"308","author":"Nimmerjahn A","year":"2005","unstructured":"A Nimmerjahn, F Kirchhoff, F Helmchen, Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science 308, 1314\u20131318 (2005).","journal-title":"Science"},{"key":"e_1_3_4_55_2","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/nn1472","article-title":"ATP mediates rapid microglial response to local brain injury in vivo","volume":"8","author":"Davalos D","year":"2005","unstructured":"D Davalos, , ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci 8, 752\u2013758 (2005).","journal-title":"Nat Neurosci"},{"key":"e_1_3_4_56_2","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1038\/nn1805","article-title":"The P2Y12 receptor regulates microglial activation by extracellular nucleotides","volume":"9","author":"Haynes SE","year":"2006","unstructured":"SE Haynes, , The P2Y12 receptor regulates microglial activation by extracellular nucleotides. Nat Neurosci 9, 1512\u20131519 (2006).","journal-title":"Nat Neurosci"},{"key":"e_1_3_4_57_2","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1073\/pnas.1520398113","article-title":"Purinergic receptor P2RY12-dependent microglial closure of the injured blood-brain barrier","volume":"113","author":"Lou N","year":"2016","unstructured":"N Lou, , Purinergic receptor P2RY12-dependent microglial closure of the injured blood-brain barrier. Proc Natl Acad Sci USA 113, 1074\u20131079 (2016).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_58_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/1471-2105-15-293","article-title":"jvenn: An interactive Venn diagram viewer","volume":"15","author":"Bardou P","year":"2014","unstructured":"P Bardou, J Mariette, F Escudi\u00e9, C Djemiel, C Klopp, jvenn: An interactive Venn diagram viewer. BMC Bioinformatics 15, 293 (2014).","journal-title":"BMC Bioinformatics"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1525528113","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1525528113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T18:17:59Z","timestamp":1692209879000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1525528113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,16]]},"references-count":58,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,3,22]]}},"alternative-id":["10.1073\/pnas.1525528113"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1525528113","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726151340.793515827","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,16]]},"assertion":[{"value":"2016-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}