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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,6,28]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Fungal infections pose a serious threat to human health and result in several million deaths annually. To survive in their human host, pathogenic fungi require the disaccharide, trehalose. Significantly, the enzymes that synthesize trehalose are absent in humans, and thus serve as potential targets for novel antifungal intervention. Here, we describe multiple structures of one of the trehalose biosynthetic enzymes, trehalose-6-phosphate phosphatase (Tps2). These structures and germane in vivo and biochemical studies reveal the significance of the Tps2 N-terminal domain in fungal cellular stress responses and the conformational flexibility of the Tps2 C-terminal domain that imposes exquisite substrate specificity and permits efficient catalysis. These structures pave the way for \u201crational\u201d inhibitor design against Tps2, facilitating antifungal drug design.<\/jats:p>","DOI":"10.1073\/pnas.1601774113","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T00:13:39Z","timestamp":1466036019000},"page":"7148-7153","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":66,"title":["Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis"],"prefix":"10.1073","volume":"113","author":[{"given":"Yi","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Tenor","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dena L.","family":"Toffaletti","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica J.","family":"Washington","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Therapeutics, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Shadrick","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Therapeutics, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Schumacher","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Therapeutics, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Perfect","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,6,15]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"165rv13","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: Human fungal infections","volume":"4","author":"Brown GD","year":"2012","unstructured":"GD Brown, , Hidden killers: Human fungal infections. 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