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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,10,25]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Cys-loop receptors belong to a family of ion channels that are involved in fast synaptic transmission. Allosteric modulators of Cys-loop receptors hold therapeutic potential as they tweak receptor function while preserving the normal fluctuations in neurotransmitter signaling at the synapse. Here, we take advantage of a model Cys-loop receptor, the\n                    <jats:italic>Erwinia<\/jats:italic>\n                    ligand-gated ion channel (ELIC). We determined cocrystal structures of ELIC in complex with chlorpromazine (IC\n                    <jats:sub>50<\/jats:sub>\n                    , \u223c160 \u03bcM) and its brominated derivative bromopromazine, which unveil an allosteric binding site localized at the interface between the extracellular ligand-binding domain and the pore-forming transmembrane domain. Our results demonstrate that the different allosteric binding sites present in Cys-loop receptors form an almost continuous path stretching from top to bottom of the receptor.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1603101113","type":"journal-article","created":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T13:24:07Z","timestamp":1476192247000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":31,"title":["Allosteric binding site in a Cys-loop receptor ligand-binding domain unveiled in the crystal structure of ELIC in complex with chlorpromazine"],"prefix":"10.1073","volume":"113","author":[{"given":"Mieke","family":"Nys","sequence":"first","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline","family":"Wijckmans","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Farinha","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zge","family":"Yoluk","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Stockholm and Uppsala, SE-17121 Stockholm, Sweden;"},{"name":"Theoretical and Computational Biophysics, Department of Theoretical Physics, Kungliga Tekniska H\u00f6gskolan Royal Institute of Technology, SE-17121 Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Andersson","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Stockholm and Uppsala, SE-17121 Stockholm, Sweden;"},{"name":"Theoretical and Computational Biophysics, Department of Theoretical Physics, Kungliga Tekniska H\u00f6gskolan Royal Institute of Technology, SE-17121 Stockholm, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijke","family":"Brams","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radovan","family":"Spurny","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Peigneur","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Pharmacology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Pharmacology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Lindahl","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Stockholm and Uppsala, SE-17121 Stockholm, Sweden;"},{"name":"Theoretical and Computational Biophysics, Department of Theoretical Physics, Kungliga Tekniska H\u00f6gskolan Royal Institute of Technology, SE-17121 Stockholm, Sweden;"},{"name":"Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE-17121 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Ulens","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Neurobiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,10,10]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"495","article-title":"Fifty years chlorpromazine: A historical perspective","volume":"3","author":"Ban TA","year":"2007","unstructured":"TA Ban, Fifty years chlorpromazine: A historical perspective. 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