{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:33:05Z","timestamp":1792873985115,"version":"4.5.0"},"reference-count":70,"publisher":"National Academy of Sciences","issue":"35","license":[{"start":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T00:00:00Z","timestamp":1470960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/preview_site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1005A\/F"],"award-info":[{"award-number":["01KI1005A\/F"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"German Center for Infection Research","doi-asserted-by":"crossref","award":["AvB:8025801803\/RH:8000 204-3"],"award-info":[{"award-number":["AvB:8025801803\/RH:8000 204-3"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,8,30]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Severe acute respiratory syndrome coronavirus (SARS-CoV) is one of the most pathogenic human coronaviruses. Virulence is reflected in the molecular interplay between virus and host cells. Here we show a strategy of how SARS-CoV antagonizes the host antiviral factor p53, which impairs viral replication. The papain-like protease of the nonstructural protein 3 of SARS-CoV and other coronaviruses physically interact with and stabilize E3 ubiquitin ligase ring-finger and CHY zinc-finger domain-containing 1 (RCHY1), thereby augmenting RCHY1-mediated degradation of p53. The SARS-unique domain (SUD) enhances these effects. Knockout of p53 promotes replication of SARS-CoV replicons and of infectious virus. Taken together we identify cellular p53 as antiviral measure of coronavirus-infected cells, which is counteracted via the stabilization of RCHY1 by viral SUD and papain-like protease (PL\n                    <jats:sup>pro<\/jats:sup>\n                    ) proteins and via ubiquitination of p53.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1603435113","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T23:09:19Z","timestamp":1471043359000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":173,"title":["p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PL\n                    <sup>pro<\/sup>\n                    via E3 ubiquitin ligase RCHY1"],"prefix":"10.1073","volume":"113","author":[{"given":"Yue","family":"Ma-Lauer","sequence":"first","affiliation":[{"name":"Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, 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