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Here, by monitoring the pH of SVs in GABAergic neurons apart from excitatory glutamatergic neurons, we show that GABA transport into SVs is tightly coupled to SV alkalization in living neurons, which occurs at an unexpectedly slow rate, suggesting a possible contribution of incompletely filled vesicles during sustained stimulation at GABAergic synapses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1604527113","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T21:55:26Z","timestamp":1473198926000},"page":"10702-10707","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":61,"title":["Unique pH dynamics in GABAergic synaptic vesicles illuminates the mechanism and kinetics of GABA loading"],"prefix":"10.1073","volume":"113","author":[{"given":"Yoshihiro","family":"Egashira","sequence":"first","affiliation":[{"name":"Laboratory of Neural Membrane Biology, Graduate School of Brain Science, Doshisha 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