{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:36:24Z","timestamp":1781105784707,"version":"3.54.1"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"43","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,10,25]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    F-actin is assembled downstream of major actin nucleators like the actin-related proteins 2\/3 (Arp2\/3) complex and formins. Capping Protein (CP), conversely, halts actin assembly. The ankyrin-repeat protein V-1 has been shown to block CP activity in vitro. Here we show that V-1 overexpression and knockout in\n                    <jats:italic>Dictyostelium<\/jats:italic>\n                    amoeba phenocopy CP knockdown and overexpression, respectively. Moreover, V-1 must be capable of binding CP to exhibit overexpression phenotypes and rescue V-1\u2013null cells. Finally, we present evidence that V-1\u2019s ability to sequester CP is regulated by phosphorylation. Together, our data demonstrate that V-1 controls major actin-dependent cellular processes in vivo by virtue of its ability to inhibit CP and that its influence may be modulated in cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1605350113","type":"journal-article","created":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T13:24:07Z","timestamp":1476192247000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":29,"title":["V-1 regulates capping protein activity in vivo"],"prefix":"10.1073","volume":"113","author":[{"given":"Goeh","family":"Jung","sequence":"first","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4803-6542","authenticated-orcid":false,"given":"Christopher 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