{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T05:58:29Z","timestamp":1793771909249,"version":"4.6.1"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"33","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/preview_site\/misc\/userlicense.xhtml"}],"funder":[{"name":"NIH\/NCI","award":["R01CA133053"],"award-info":[{"award-number":["R01CA133053"]}]},{"name":"NIH\/NCI","award":["P50CA098252"],"award-info":[{"award-number":["P50CA098252"]}]},{"name":"NIH\/NCI","award":["U19AI113212"],"award-info":[{"award-number":["U19AI113212"]}]},{"name":"NIH\/NCI","award":["RO1 CA83679"],"award-info":[{"award-number":["RO1 CA83679"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,8,16]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    O-linked GlcNAcylation is a reversible posttranslational protein modification on serine or threonine residues and regulates multiple cellular signaling pathways. We discovered elevated O-GlcNAc and O-GlcNAc transferase (OGT) in HPV-associated cervical neoplasms relative to the normal cervix. We show that HPV\n                    <jats:italic>E6<\/jats:italic>\n                    upregulates OGT, increases O-GlcNAc, stabilizes c-MYC via O-GlcNAc, and enhances HPV oncogene activities. Conversely, suppression of O-GlcNAc in HPV-transformed cells by knocking down or inhibiting OGT impairs HPV oncogene-induced activities and impedes tumor growth in animal models. Thus, O-GlcNAc plays a critical role in HPV-induced carcinogenesis, and targeting O-GlcNAc might prove to be a potential therapeutic approach.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1606801113","type":"journal-article","created":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T23:24:06Z","timestamp":1470093846000},"page":"9333-9338","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":77,"title":["O-linked GlcNAcylation elevated by HPV E6 mediates viral oncogenesis"],"prefix":"10.1073","volume":"113","author":[{"given":"Qinghua","family":"Zeng","sequence":"first","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"},{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Xun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yining","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Dong","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Long","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Shi","family":"Quan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Shu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Xian","family":"Zhai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Youssef","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutao","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA 02118;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyong","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, University of Texas MD Anderson Cancer Center, Houston, TX 77030;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Genovese","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise T.","family":"Chow","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Xiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China;"},{"name":"Division of Hematology and Oncology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.11.046"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2010.02624.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.09.046"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060608-102511"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3334"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2013.844093"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3114"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11742"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12537"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06668"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4417"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.32.18961"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.03.001"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.11.021"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.470047"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1470"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10656"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00099"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.04.026"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.06.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2886"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0555217"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.025288"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-0549"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3560247"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4283-4296.2001"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980925)78:1<41::AID-IJC8>3.0.CO;2-X"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-1271"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjv009"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a014241"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a014373"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.15.7198-7201.2001"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4467-4472.2001"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.18.9852-9861.2003"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01318-08"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600017200"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1110"},{"key":"e_1_3_3_39_2","first-page":"3991","article-title":"Association of p27Kip1, cyclin E and c-myc expression with progression and prognosis in HPV-positive cervical neoplasms","volume":"18","author":"Dellas A","year":"1998","unstructured":"A Dellas, E Schultheiss, MR Leivas, H Moch, J Torhorst, Association of p27Kip1, cyclin E and c-myc expression with progression and prognosis in HPV-positive cervical neoplasms. Anticancer Res 18, 3991\u20133998 (1998).","journal-title":"Anticancer Res"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1606801113","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1606801113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T01:24:01Z","timestamp":1649813041000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1606801113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":39,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2016,8,16]]}},"alternative-id":["10.1073\/pnas.1606801113"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1606801113","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8]]},"assertion":[{"value":"2016-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}