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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,7,19]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The essence of the adaptive immune response depends on the specificity of antigen receptors. This report identifies shared alpha\u2013beta T-cell receptor (TCR) pairs from the tissues of HLA-A2\n                    <jats:sup>+<\/jats:sup>\n                    patients with breast cancer and control donors. Using an emulsion-based RT-PCR assay, we analyzed TCR sequences from tissues ex vivo. We identified multiple TCR pairs shared between tumors, but not control samples. Although recent reports have concluded that anticancer T-cell responses depend on patient-specific mutation-associated neoantigens, this study provides evidence that T cells also recognize shared antigens. This approach has broad application to a variety of research questions where the end goal is to examine T-cell repertoires and\/or identify T-cell antigens.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1606994113","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T00:13:39Z","timestamp":1466036019000},"page":"8272-8277","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR"],"prefix":"10.1073","volume":"113","author":[{"given":"Daniel J.","family":"Munson","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colt A.","family":"Egelston","sequence":"additional","affiliation":[{"name":"Department of Immuno-Oncology, Beckman Research 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