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Here, using X-ray crystallography, computational energy calculations, isothermal titration calorimetry, glycan-binding assays, and MuV glycoprotein-mediated cell fusion assays, we show that the trisaccharide containing an \u03b12,3-linked sialic acid on the cell surface acts as a receptor for MuV. In conjunction with elucidation of the distribution of structurally different sialylated glycans in tissues and organs, this finding will lead to a better understanding of MuV tropism and pathogenesis.<\/jats:p>","DOI":"10.1073\/pnas.1608383113","type":"journal-article","created":{"date-parts":[[2016,9,26]],"date-time":"2016-09-26T20:48:35Z","timestamp":1474922915000},"page":"11579-11584","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":112,"title":["Trisaccharide containing \u03b12,3-linked sialic acid is a receptor for mumps virus"],"prefix":"10.1073","volume":"113","author":[{"given":"Marie","family":"Kubota","sequence":"first","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumpei","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rei","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Kohda","sequence":"additional","affiliation":[{"name":"Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichi","family":"Nakakita","sequence":"additional","affiliation":[{"name":"Department of Functional Glycomics, Life Science Research Center, Kagawa University, Kagawa 761-0793, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Hiramatsu","sequence":"additional","affiliation":[{"name":"Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Health Science Hills, College of Life and Health Sciences, Chubu University, Kasugai 487-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Infection, Kitasato Institute for Life Sciences, Tokyo 108-8641, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutaka","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Photon Factory, Institute of Materials Structure Science, High-Energy Accelerator Research Organization, Tsukuba 305-0801, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsunori","family":"Shiroishi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Yanagi","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Hashiguchi","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,9,26]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"1024","volume-title":"Fields Virology","author":"Rubin SA","year":"2013","unstructured":"SA Rubin, CJ Sauder, KM Carbone, Mumps virus. 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J Virol 85, 11038\u201311047 (2011).","journal-title":"J Virol"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/nature11741","article-title":"Functional and evolutionary insight from the crystal structure of rubella virus protein E1","volume":"493","author":"DuBois RM","year":"2013","unstructured":"RM DuBois, , Functional and evolutionary insight from the crystal structure of rubella virus protein E1. Nature 493, 552\u2013556 (2013).","journal-title":"Nature"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"e1004791","DOI":"10.1371\/journal.ppat.1004791","article-title":"Remembering mumps","volume":"11","author":"Latner DR","year":"2015","unstructured":"DR Latner, CJ Hickman, Remembering mumps. 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