{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:52:15Z","timestamp":1790423535897,"version":"4.1.0"},"reference-count":59,"publisher":"National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2017,8,6]],"date-time":"2017-08-06T00:00:00Z","timestamp":1501977600000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI AI094991"],"award-info":[{"award-number":["R01 AI AI094991"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,2,21]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bordetella<\/jats:italic>\n                    spp. includes\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    , the causal agent of whooping cough. The\n                    <jats:italic>Bordetella<\/jats:italic>\n                    virulence gene (BvgAS) two-component regulatory system (TCS) is considered the \u201cmaster virulence regulator\u201d in\n                    <jats:italic>Bordetella<\/jats:italic>\n                    , as it controls expression of all known virulence factor-encoding genes. We show here that another TCS, PlrSR, is required for BvgAS activity in the lower respiratory tract (LRT) and for virulence even when BvgAS is rendered constitutively active, suggesting that it controls critical functions for bacterial survival in the LRT independently of BvgAS. Our data introduce a new layer of complexity to a paradigm of\n                    <jats:italic>Bordetella<\/jats:italic>\n                    virulence control that has held for more than 30 y, and they indicate the existence of previously unknown bacterial factors that may serve as vaccine components and therapeutic targets.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1609565114","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T21:05:30Z","timestamp":1486415130000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["<i>Bordetella<\/i>\n                    PlrSR regulatory system controls BvgAS activity and virulence in the lower respiratory tract"],"prefix":"10.1073","volume":"114","author":[{"given":"M. Ashley","family":"Bone","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron J.","family":"Wilk","sequence":"additional","affiliation":[{"name":"Department of Biology, Westmont College, Santa Barbara, CA 93108;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew I.","family":"Perault","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara A.","family":"Marlatt","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich 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