{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:13:11Z","timestamp":1793106791472,"version":"4.5.0"},"reference-count":63,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,8,14]]},"abstract":"<jats:p>\n                    Overexpression of the zinc enzyme carbonic anhydrase (CA; EC\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"ec\" xlink:href=\"4.2.1.1\">4.2.1.1<\/jats:ext-link>\n                    ) XII is observed in certain human cancers. This bitopic\n membrane protein contains an N-terminal extracellular catalytic domain,\n a membrane-spanning \u03b1-helix, and a small intracellular C-terminal\n domain. We have determined the three-dimensional structure of the\n extracellular catalytic domain of human CA XII by x-ray\n crystallographic methods at 1.55-\u212b resolution. The structure reveals a\n prototypical CA fold; however, two CA XII domains associate to form an\n isologous dimer, an observation that is confirmed by studies of the\n enzyme in solution. The identification of signature GXXXG and GXXXS\n motifs in the transmembrane sequence that facilitate helix\u2013helix\n association is additionally consistent with dimeric architecture. The\n dimer interface is situated so that the active site clefts of each\n monomer are clearly exposed on one face of the dimer, and the C termini\n are located together on the opposite face of the dimer to facilitate\n membrane interaction. The amino acid composition of the active-site\n cleft closely resembles that of the other CA isozymes in the immediate\n vicinity of the catalytic zinc ion, but differs in the region of the\n nearby \u03b1-helical \u201c130's segment.\u201d The structure of the CA\n XII\u2013acetazolamide complex is also reported at 1.50-\u212b resolution, and\n prospects for the design of CA XII-specific inhibitors of possible\n chemotherapeutic value are discussed.\n                  <\/jats:p>","DOI":"10.1073\/pnas.161301298","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:10Z","timestamp":1027679650000},"page":"9545-9550","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":318,"title":["Crystal structure of the dimeric extracellular domain of human carbonic anhydrase XII, a bitopic membrane protein overexpressed in certain cancer tumor cells"],"prefix":"10.1073","volume":"98","author":[{"given":"Douglas A.","family":"Whittington","sequence":"first","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Waheed","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Ulmasov","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gul N.","family":"Shah","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey H.","family":"Grubb","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William S.","family":"Sly","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Christianson","sequence":"additional","affiliation":[{"name":"Roy and Diana Vagelos Laboratories, Department of Chemistry,\r University of Pennsylvania, Philadelphia, PA 19104-6323; and\r Edward A. Doisy Department of Biochemistry and Molecular\r Biology, St. Louis University School of Medicine, St. Louis, MO 63104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,8,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-0348-8446-4","volume-title":"The Carbonic Anhydrases","author":"Chegwidden W R","year":"2000","unstructured":"W R Chegwidden, N D Carter, Y H Edwards The Carbonic Anhydrases (Birkh\u00e4user, Boston, 2000)."},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1016\/S0021-9258(18)99413-5","volume":"242","author":"Coleman J E","year":"1967","unstructured":"J E Coleman J Biol Chem 242, 5212\u20135219 (1967).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1073\/pnas.70.9.2505","volume":"70","author":"Lindskog S","year":"1973","unstructured":"S Lindskog, J E Coleman Proc Natl Acad Sci USA 70, 2505\u20132508 (1973).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1021\/ar00145a005","volume":"21","author":"Silverman D N","year":"1988","unstructured":"D N Silverman, S Lindskog Acc Chem Res 21, 30\u201336 (1988).","journal-title":"Acc Chem Res"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/0959-440X(93)90156-F","volume":"3","author":"Lindskog S","year":"1993","unstructured":"S Lindskog, A Liljas Curr Opin Struct Biol 3, 915\u2013920 (1993).","journal-title":"Curr Opin Struct Biol"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1021\/ar9501232","volume":"29","author":"Christianson D W","year":"1996","unstructured":"D W Christianson, C A Fierke Acc Chem Res 29, 331\u2013339 (1996).","journal-title":"Acc Chem Res"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1146\/annurev.bi.64.070195.002111","volume":"64","author":"Sly W S","year":"1995","unstructured":"W S Sly, P Y Hu Annu Rev Biochem 64, 375\u2013401 (1995).","journal-title":"Annu Rev Biochem"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"7417","DOI":"10.1073\/pnas.89.16.7417","volume":"89","author":"Krueger N X","year":"1992","unstructured":"N X Krueger, H Saito Proc Natl Acad Sci USA 89, 7417\u20137421 (1992).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_9_2","first-page":"1497","volume":"13","author":"Barnea G","year":"1993","unstructured":"G Barnea, O Silvennoinen, B Shaanan, A M Honegger, P D Canoll, P D'Eustachio, B Morse, J B Levy, S LaForgia, K Huebner, et al. Mol Cell Biol 13, 1497\u20131506 (1993).","journal-title":"Mol Cell Biol"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"10573","DOI":"10.1016\/S0021-9258(18)82237-2","volume":"268","author":"Levy J B","year":"1993","unstructured":"J B Levy, P D Canoll, O Silvennoinen, G Barnea, B Morse, A M Honegger, J-T Huang, L A Cannizzaro, S-H Park, T Druck, et al. J Biol Chem 268, 10573\u201310581 (1993).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0092-8674(95)90312-7","volume":"82","author":"Peles E","year":"1995","unstructured":"E Peles, M Nativ, P L Campbell, T Sakurai, R Martinez, S Lev, D O Clary, J Schilling, G Barnea, G D Plowman, et al. Cell 82, 251\u2013260 (1995).","journal-title":"Cell"},{"key":"e_1_3_4_12_2","unstructured":"Torczynski R. M. & Bollon A. P. (1996) U.S. Patent\n 5 589 579."},{"key":"e_1_3_4_13_2","first-page":"2362","volume":"57","author":"McKiernan J M","year":"1997","unstructured":"J M McKiernan, R Buttyan, N H Bander, M D Stifelman, A E Katz, M-W Chen, C A Olsson, I S Sawczuk Cancer Res 57, 2362\u20132365 (1997).","journal-title":"Cancer Res"},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/ijc.2910540218","volume":"54","author":"Zavad\u00e1 J","year":"1993","unstructured":"J Zavad\u00e1, Z Z\u00e1vadov\u00e1, S Pastorekov\u00e1, F Ciampor, J Pastorek, V Zeln\u00edk Int J Cancer 54, 268\u2013274 (1993).","journal-title":"Int J Cancer"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"7608","DOI":"10.1073\/pnas.95.13.7608","volume":"95","author":"T\u00fcreci O","year":"1998","unstructured":"O T\u00fcreci, U Sahin, E Vollmar, S Siemer, E G\u00f6ttert, G Seitz, A-K Parkkila, G N Shah, J H Grubb, M Pfreundschuh, et al. Proc Natl Acad Sci USA 95, 7608\u20137613 (1998).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","first-page":"12596","DOI":"10.1073\/pnas.95.21.12596","volume":"95","author":"Ivanov S V","year":"1998","unstructured":"S V Ivanov, I Kuzmin, M-H Wei, S Pack, L Geil, B E Johnson, E J Stanbridge, M I Lerman Proc Natl Acad Sci USA 95, 12596\u201312601 (1998).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1126\/science.8493574","volume":"260","author":"Latif F","year":"1993","unstructured":"F Latif, K Tory, J Gnarra, M Yao, F-M Duh, M L Orcutt, T Stackhouse, I Kuzmin, W Modi, L Geil, et al. Science 260, 1317\u20131320 (1993).","journal-title":"Science"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"14212","DOI":"10.1073\/pnas.97.26.14212","volume":"97","author":"Ulmasov B","year":"2000","unstructured":"B Ulmasov, A Waheed, G N Shah, J H Grubb, W S Sly, C Tu, D N Silverman Proc Natl Acad Sci USA 97, 14212\u201314217 (2000).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_19_2","first-page":"2877","volume":"9","author":"Pastorek J","year":"1994","unstructured":"J Pastorek, S Pastorekov\u00e1, I Callebaut, J P Mornon, V Zelnik, R Opavsky, M Zat'ovicov\u00e1, S Liao, D Portetelle, E J Stanbridge, et al. Oncogene 9, 2877\u20132888 (1994).","journal-title":"Oncogene"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1006\/geno.1996.0223","volume":"33","author":"Opavsk\u00fd R","year":"1996","unstructured":"R Opavsk\u00fd, S Pastorekov\u00e1, V Zeln\u00edk, A Gibadulinov\u00e1, E J Stanbridge, J Z\u00e1vada, R Kettmann, J Pastorek Genomics 33, 480\u2013487 (1996).","journal-title":"Genomics"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"8795","DOI":"10.1016\/S0021-9258(19)38958-6","volume":"265","author":"Zhu X L","year":"1990","unstructured":"X L Zhu, W S Sly J Biol Chem 265, 8795\u20138801 (1990).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/BF00121214","volume":"14","author":"Mart\u00ednez-Zaguil\u00e1n R","year":"1996","unstructured":"R Mart\u00ednez-Zaguil\u00e1n, E A Seftor, R E B Seftor, Y-W Chu, R J Gillies, M J C Hendrix Clin Exp Metastasis 14, 176\u2013186 (1996).","journal-title":"Clin Exp Metastasis"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1073\/pnas.040554897","volume":"97","author":"Parkkila S","year":"2000","unstructured":"S Parkkila, H Rajaniemi, A-K Parkkila, J Kivel\u00e4, A Waheed, S Pastorekov\u00e1, J Pastorek, W S Sly Proc Natl Acad Sci USA 97, 2220\u20132224, . (First Published February 25, 2000; 10.1073\/pnas.040554897). (2000).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_24_2","first-page":"125","volume":"38","author":"Sundaram V","year":"1986","unstructured":"V Sundaram, P Rumbolo, J H Grubb, P Strisciuglio, W S Sly Am J Hum Genet 38, 125\u2013136 (1986).","journal-title":"Am J Hum Genet"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli U K","year":"1970","unstructured":"U K Laemmli Nature (London) 227, 680\u2013685 (1970).","journal-title":"Nature (London)"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","volume":"276","author":"Otwinowski Z","year":"1997","unstructured":"Z Otwinowski, W Minor Methods Enzymol 276, 307\u2013326 (1997).","journal-title":"Methods Enzymol"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1021\/bi002295z","volume":"40","author":"Duda D","year":"2001","unstructured":"D Duda, C Tu, M Qian, P Laipis, M Agbandje-McKenna, D N Silverman, R McKenna Biochemistry 40, 1741\u20131748 (2001).","journal-title":"Biochemistry"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1107\/S0108767393007597","volume":"50","author":"Navaza J","year":"1994","unstructured":"J Navaza Acta Crystallogr A 50, 157\u2013163 (1994).","journal-title":"Acta Crystallogr A"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","volume":"50","year":"1994","unstructured":"Acta Crystallogr D 50, 760\u2013763 (1994).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1107\/S0108767390010224","volume":"47","author":"Jones T A","year":"1991","unstructured":"T A Jones, J-Y Zou, S W Cowan, M Kjeldgaard Acta Crystallogr A 47, 110\u2013119 (1991).","journal-title":"Acta Crystallogr A"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","volume":"54","author":"Br\u00fcnger A T","year":"1998","unstructured":"A T Br\u00fcnger, P D Adams, G M Clore, W L DeLano, P Gros, R W Grosse-Kunstleve, J-S Jiang, J Kuszewski, M Nilges, N S Pannu, et al. Acta Crystallogr D 54, 905\u2013921 (1998).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1107\/S0021889891004399","volume":"24","author":"Kraulis P J","year":"1991","unstructured":"P J Kraulis J Appl Crystallogr 24, 946\u2013950 (1991).","journal-title":"J Appl Crystallogr"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/S1093-3263(97)00021-1","volume":"15","author":"Esnouf R M","year":"1997","unstructured":"R M Esnouf J Mol Graphics 15, 132\u2013134 (1997).","journal-title":"J Mol Graphics"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1107\/S0907444994006396","volume":"50","author":"Merritt E A","year":"1994","unstructured":"E A Merritt, M E P Murphy Acta Crystallogr D 50, 869\u2013873 (1994).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"13589","DOI":"10.1073\/pnas.93.24.13589","volume":"93","author":"Stams T","year":"1996","unstructured":"T Stams, S K Nair, T Okuyama, A Waheed, W S Sly, D W Christianson Proc Natl Acad Sci USA 93, 13589\u201313594 (1996).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/0022-2836(90)90333-H","volume":"214","author":"Merz K M","year":"1990","unstructured":"K M Merz J Mol Biol 214, 799\u2013802 (1990).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1038\/328834a0","volume":"328","author":"Miller S","year":"1987","unstructured":"S Miller, A M Lesk, J Janin, C Chothia Nature (London) 328, 834\u2013836 (1987).","journal-title":"Nature (London)"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/protein\/13.2.77","volume":"13","author":"Jones S","year":"2000","unstructured":"S Jones, A Marin, J M Thornton Protein Eng 13, 77\u201382 (2000).","journal-title":"Protein Eng"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/1097-0134(20001001)41:1<47::AID-PROT80>3.0.CO;2-8","volume":"41","author":"Ponstingl H","year":"2000","unstructured":"H Ponstingl, K Henrick, J M Thornton Proteins Struct Funct Genet 41, 47\u201357 (2000).","journal-title":"Proteins Struct Funct Genet"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1006\/jmbi.1999.3489","volume":"296","author":"Russ W P","year":"2000","unstructured":"W P Russ, D M Engelman J Mol Biol 296, 911\u2013919 (2000).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1006\/jmbi.1999.3488","volume":"296","author":"Senes A","year":"2000","unstructured":"A Senes, M Gerstein, D M Engelman J Mol Biol 296, 921\u2013936 (2000).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1126\/science.276.5309.131","volume":"276","author":"MacKenzie K R","year":"1997","unstructured":"K R MacKenzie, J H Prestegard, D M Engelman Science 276, 131\u2013133 (1997).","journal-title":"Science"},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.1021\/bi010357v","volume":"40","author":"Smith S O","year":"2001","unstructured":"S O Smith, D Song, S Shekar, M Groesbeek, M Ziliox, S Aimoto Biochemistry 40, 6553\u20136558 (2001).","journal-title":"Biochemistry"},{"key":"e_1_3_4_44_2","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1006\/jmbi.1999.3310","volume":"294","author":"Blom N","year":"1999","unstructured":"N Blom, S Gammeltoft, S Brunak J Mol Biol 294, 1351\u20131362 (1999).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_45_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/prot.340150110","volume":"15","author":"Xue Y","year":"1993","unstructured":"Y Xue, J Vidgren, L A Svensson, A Liljas, B-H Jonsson, S Lindskog Proteins Struct Funct Genet 15, 80\u201387 (1993).","journal-title":"Proteins Struct Funct Genet"},{"key":"e_1_3_4_46_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/prot.340170112","volume":"17","author":"Xue Y","year":"1993","unstructured":"Y Xue, A Liljas, B-H Jonsson, S Lindskog Proteins Struct Funct Genet 17, 93\u2013106 (1993).","journal-title":"Proteins Struct Funct Genet"},{"key":"e_1_3_4_47_2","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/0022-2836(92)90531-N","volume":"227","author":"H\u00e5kansson K","year":"1992","unstructured":"K H\u00e5kansson, M Carlsson, L A Svensson, A Liljas J Mol Biol 227, 1192\u20131204 (1992).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_48_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1107\/S0907444993009667","volume":"50","author":"H\u00e5kansson K","year":"1994","unstructured":"K H\u00e5kansson, C Briand, V Zaitsev, Y Xue, A Liljas Acta Crystallogr D 50, 101\u2013104 (1994).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_49_2","doi-asserted-by":"crossref","first-page":"9455","DOI":"10.1021\/ja00025a005","volume":"113","author":"Nair S K","year":"1991","unstructured":"S K Nair, D W Christianson J Am Chem Soc 113, 9455\u20139458 (1991).","journal-title":"J Am Chem Soc"},{"key":"e_1_3_4_50_2","doi-asserted-by":"crossref","first-page":"7913","DOI":"10.1021\/bi00445a054","volume":"28","author":"Tu C","year":"1989","unstructured":"C Tu, D N Silverman, C Forsman, B-H Jonsson, S Lindskog Biochemistry 28, 7913\u20137918 (1989).","journal-title":"Biochemistry"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.1021\/ja00091a006","volume":"116","author":"Cappalonga Bunn A M","year":"1994","unstructured":"A M Cappalonga Bunn, R S Alexander, D W Christianson J Am Chem Soc 116, 5063\u20135068 (1994).","journal-title":"J Am Chem Soc"},{"key":"e_1_3_4_52_2","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1002\/pro.5560071201","volume":"7","author":"Boriack-Sjodin P A","year":"1998","unstructured":"P A Boriack-Sjodin, S Zeitlin, H-H Chen, L Crenshaw, S Gross, A Dantanarayana, P Delgado, J A May, T Dean, D W Christianson Protein Sci 7, 2483\u20132489 (1998).","journal-title":"Protein Sci"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"12125","DOI":"10.1021\/ja002627n","volume":"122","author":"Kim C-Y","year":"2000","unstructured":"C-Y Kim, J S Chang, J B Doyon, T T Baird, C A Fierke, A Jain, D W Christianson J Am Chem Soc 122, 12125\u201312134 (2000).","journal-title":"J Am Chem Soc"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1002\/pro.5560070303","volume":"7","author":"Stams T","year":"1998","unstructured":"T Stams, Y Chen, P A Boriack-Sjodin, J D Hurt, J Liao, J A May, T Dean, P Laipis, D N Silverman, D W Christianson Protein Sci 7, 556\u2013563 (1998).","journal-title":"Protein Sci"},{"key":"e_1_3_4_55_2","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1021\/bi00012a016","volume":"34","author":"Nair S K","year":"1995","unstructured":"S K Nair, J F Krebs, D W Christianson, C A Fierke Biochemistry 34, 3981\u20133989 (1995).","journal-title":"Biochemistry"},{"key":"e_1_3_4_56_2","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/0141-8130(90)90040-H","volume":"12","author":"Vidgren J","year":"1990","unstructured":"J Vidgren, A Liljas, N P C Walker Intl J Biol Macromol 12, 342\u2013344 (1990).","journal-title":"Intl J Biol Macromol"},{"key":"e_1_3_4_57_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/BF02704000","volume":"8","author":"Chakravarty S","year":"1985","unstructured":"S Chakravarty, V S Yadava, V Kumar, K K Kannan J Biosci 8, 491\u2013498 (1985).","journal-title":"J Biosci"},{"key":"e_1_3_4_58_2","doi-asserted-by":"crossref","first-page":"10949","DOI":"10.1073\/pnas.92.24.10949","volume":"92","author":"Boriack-Sjodin P A","year":"1995","unstructured":"P A Boriack-Sjodin, R W Heck, P J Laipis, D N Silverman, D W Christianson Proc Natl Acad Sci USA 92, 10949\u201310953 (1995).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_59_2","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.1016\/S0021-9258(18)62326-9","volume":"246","author":"Khalifah R G","year":"1971","unstructured":"R G Khalifah J Biol Chem 246, 2561\u20132573 (1971).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_60_2","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1021\/bi962663s","volume":"36","author":"Baird T T","year":"1997","unstructured":"T T Baird, A Waheed, T Okuyama, W S Sly, C A Fierke Biochemistry 36, 2669\u20132678 (1997).","journal-title":"Biochemistry"},{"key":"e_1_3_4_61_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1749-6632.1984.tb12314.x","volume":"429","author":"Kannan K K","year":"1984","unstructured":"K K Kannan, M Ramanadham, T A Jones Ann NY Acad Sci 429, 49\u201360 (1984).","journal-title":"Ann NY Acad Sci"},{"key":"e_1_3_4_62_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0014-5793(00)02022-6","volume":"482","author":"Mallis R J","year":"2000","unstructured":"R J Mallis, B W Poland, T K Chatterjee, R A Fisher, S Darmawan, R B Honzatko, J A Thomas FEBS Lett 482, 237\u2013241 (2000).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_63_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/prot.340110407","volume":"11","author":"Nicholls A","year":"1991","unstructured":"A Nicholls, K A Sharp, B Honig Proteins Struct Funct Genet 11, 281\u2013296 (1991).","journal-title":"Proteins Struct Funct Genet"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.161301298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T01:25:09Z","timestamp":1654565109000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.161301298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8,7]]},"references-count":63,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2001,8,14]]}},"alternative-id":["10.1073\/pnas.161301298"],"URL":"https:\/\/doi.org\/10.1073\/pnas.161301298","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8,7]]},"assertion":[{"value":"2001-06-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-08-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}