{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:10:20Z","timestamp":1791007820615,"version":"4.1.0"},"reference-count":71,"publisher":"National Academy of Sciences","issue":"50","license":[{"start":{"date-parts":[[2017,5,28]],"date-time":"2017-05-28T00:00:00Z","timestamp":1495929600000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AG002132"],"award-info":[{"award-number":["AG002132"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AG031220"],"award-info":[{"award-number":["AG031220"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AG010770"],"award-info":[{"award-number":["AG010770"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002336","name":"Daiichi Sankyo Company","doi-asserted-by":"publisher","award":["000"],"award-info":[{"award-number":["000"]}],"id":[{"id":"10.13039\/501100002336","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100003896","name":"Henry M. Jackson Foundation","doi-asserted-by":"publisher","award":["000"],"award-info":[{"award-number":["000"]}],"id":[{"id":"10.13039\/100003896","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001068","name":"Dana Foundation","doi-asserted-by":"publisher","award":["000"],"award-info":[{"award-number":["000"]}],"id":[{"id":"10.13039\/100001068","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001642","name":"Glenn Foundation for Medical Research","doi-asserted-by":"publisher","award":["000"],"award-info":[{"award-number":["000"]}],"id":[{"id":"10.13039\/100001642","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Rainwater Charitable Foundation","award":["000"],"award-info":[{"award-number":["000"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,12,13]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>The progressive nature of neurodegenerative diseases is due to the spread of prions, misfolded infectious proteins, in the brain. In tauopathies, the protein tau misfolds, causing several diseases, including Alzheimer\u2019s disease (AD) and chronic traumatic encephalopathy (CTE). Here we created a panel of mammalian cell lines expressing a fragment of tau fused to yellow fluorescent protein. Each cell line selectively detects tau prions that are misfolded into self-propagating conformations; such cells permit identification of minute differences among tauopathies. For example, tau prions in AD and CTE are distinct from prions in other tauopathies such as Pick\u2019s disease and progressive supranuclear palsy. These insights are likely to contribute to the development of future therapeutics.<\/jats:p>","DOI":"10.1073\/pnas.1616344113","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T21:51:19Z","timestamp":1480369879000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":176,"title":["Tau prions from Alzheimer\u2019s disease and chronic traumatic encephalopathy patients propagate in cultured cells"],"prefix":"10.1073","volume":"113","author":[{"given":"Amanda L.","family":"Woerman","sequence":"first","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"},{"name":"Department of Neurology, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Aoyagi","sequence":"additional","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"},{"name":"Daiichi Sankyo Co., Ltd., Tokyo 140-8710, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smita","family":"Patel","sequence":"additional","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabeen A.","family":"Kazmi","sequence":"additional","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Lobach","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea T.","family":"Grinberg","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of California, San Francisco, CA 94143;"},{"name":"Department of Pathology, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann C.","family":"McKee","sequence":"additional","affiliation":[{"name":"Chronic Traumatic Encephalopathy Program, Alzheimer\u2019s Disease Center, Boston University School of Medicine, Boston, MA 02118;"},{"name":"Department of Neurology, Boston University School of Medicine, Boston, MA 02118;"},{"name":"Department of Pathology, Boston University School of Medicine, Boston, MA 02118;"},{"name":"Veterans Affairs Boston Healthcare System, US Department of Veterans Affairs, Jamaica Plain, MA 02130;"},{"name":"US Department of Veterans Affairs Medical Center, Bedford, MA 01730;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William W.","family":"Seeley","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of California, San Francisco, CA 94143;"},{"name":"Department of Pathology, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven H.","family":"Olson","sequence":"additional","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"},{"name":"Department of Neurology, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley B.","family":"Prusiner","sequence":"additional","affiliation":[{"name":"Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, CA 94143;"},{"name":"Department of Neurology, University of California, San Francisco, CA 94143;"},{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2016,11,28]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)92134-3"},{"key":"e_1_3_3_2_2","first-page":"229","article-title":"Mise en \u00e9vidence immunologique de la prot\u00e9ine tau au niveau des l\u00e9sions de d\u00e9g\u00e9n\u00e9rescence neurofibrillaire de la maladie d\u2019Alzheimer","volume":"95","author":"Brion J-P","year":"1985","unstructured":"J-P Brion, H Passareiro, J Nunez, J Flament-Durand, Mise en \u00e9vidence immunologique de la prot\u00e9ine tau au niveau des l\u00e9sions de d\u00e9g\u00e9n\u00e9rescence neurofibrillaire de la maladie d\u2019Alzheimer. Arch Biol (Liege) 95, 229\u2013235. French (1985).","journal-title":"Arch Biol (Liege)"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.13.4913"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.11.4044"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.11.4040"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.12.4506"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00304630"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.4668"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlw026"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/31508"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410430617"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-004-0489-x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/349704a0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)92212-9"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03390.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90117-V"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00158a027"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199602000-00004"},{"key":"e_1_3_3_19_2","first-page":"1496","article-title":"Ultrastructure and biochemical composition of paired helical filaments in corticobasal degeneration","volume":"145","author":"Ksiezak-Reding H","year":"1994","unstructured":"H Ksiezak-Reding, , Ultrastructure and biochemical composition of paired helical filaments in corticobasal degeneration. Am J Pathol 145, 1496\u20131508 (1994).","journal-title":"Am J Pathol"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296367"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81106-X"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5395.1914"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.10.5598"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010000330"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10962"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(73)90024-8"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.39201"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1439789"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5295.2079"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110711-155524"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.04.047"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1513426112"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/2654"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/alzrt234"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10222"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1515-z"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/197192b0"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90168-X"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1514475112"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2642-12.2013"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1458-4"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1231-5"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1255555"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301175110"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.346072"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(00)00019-9"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi2004685"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.148460"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1901"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-014-1254-6"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.01.010"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90134-Z"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.19.7475"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.12.5690"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1509-x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.364067"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24517"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom6010007"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2016.01.003"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-011-0893-0"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4067"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04509"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1928.02700150029009"},{"key":"e_1_3_3_64_2","first-page":"295","article-title":"Combat wounds in operation Iraqi Freedom and operation Enduring Freedom","volume":"64","author":"Owens BD","year":"2008","unstructured":"BD Owens, , Combat wounds in operation Iraqi Freedom and operation Enduring Freedom. J Trauma 64, 295\u2013299 (2008).","journal-title":"J Trauma"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa072972"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1097\/HTR.0b013e31819581d8"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(16)30057-6"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws307"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0612-2"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhr004"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199501000-00006"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1616344113","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1616344113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:36:09Z","timestamp":1649817369000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1616344113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,28]]},"references-count":71,"journal-issue":{"issue":"50","published-print":{"date-parts":[[2016,12,13]]}},"alternative-id":["10.1073\/pnas.1616344113"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1616344113","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,28]]},"assertion":[{"value":"2016-11-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}