{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:16:47Z","timestamp":1792721807311,"version":"4.5.0"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"23","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["1016\/5-1"],"award-info":[{"award-number":["1016\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["1016\/8-1"],"award-info":[{"award-number":["1016\/8-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,6,6]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Loss of podocyte adhesion is a hallmark of glomerular disease progression. Here we unravel the in vivo composition of the podocyte adhesion machinery by the use of quantitative proteomics and identify the FERM domain protein EPB41L5 as a selectively enriched novel podocyte focal adhesion protein. EPB41L5 is essential to maintaining podocyte adhesion in vivo by recruiting the Rho GEF ARHGEF18, initiating a signaling cascade and ultimately resulting in increased actomyosin activity and focal adhesion stabilization. As EPB41L5 is down-regulated in various glomerular pathologies, these findings offer a perspective for interventions aiming to prevent loss of podocytes in glomerular disease.<\/jats:p>","DOI":"10.1073\/pnas.1617004114","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T20:50:16Z","timestamp":1495572616000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":67,"title":["The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier"],"prefix":"10.1073","volume":"114","author":[{"given":"Christoph","family":"Schell","sequence":"first","affiliation":[{"name":"Institute of Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Rogg","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Suhm","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Helmst\u00e4dter","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Sellung","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mako","family":"Yasuda-Yamahara","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"Department of Medicine, Shiga University of Medical Science, Otsu, Shiga 522-8522, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Kretz","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"BIOSS Centre for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, 79106 Freiburg, Germany;"},{"name":"III. Medizinische Klinik, Universit\u00e4tsklinikum Hamburg-Eppendorf, 20251 Hamburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"K\u00fcttner","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Suleiman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Washington University in St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laxmikanth","family":"Kollipara","sequence":"additional","affiliation":[{"name":"Leibniz-Institut f\u00fcr Analytische Wissenschaften \u2013 ISAS \u2013 e.V., 44139 Dortmund, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 P.","family":"Zahedi","sequence":"additional","affiliation":[{"name":"Leibniz-Institut f\u00fcr Analytische Wissenschaften \u2013 ISAS \u2013 e.V., 44139 Dortmund, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Sickmann","sequence":"additional","affiliation":[{"name":"Leibniz-Institut f\u00fcr Analytische Wissenschaften \u2013 ISAS \u2013 e.V., 44139 Dortmund, Germany;"},{"name":"Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen AB24 3FX, Scotland, United Kingdom;"},{"name":"Medizinische Proteom-Center, Ruhr-Universit\u00e4t Bochum, 44801 Bochum, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Eimer","sequence":"additional","affiliation":[{"name":"BIOSS Centre for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, 79106 Freiburg, Germany;"},{"name":"Center for Biological Systems Analysis (ZBSA) and Freiburg Institute for Advanced Studies, Albert-Ludwigs-University, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey S.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Pathology, Washington University in St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albrecht","family":"Kramer-Zucker","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Hirano-Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory for Vertebrate Body Plan, Center for Developmental Biology, RIKEN Kobe, Kobe 650-0047, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaya","family":"Abe","sequence":"additional","affiliation":[{"name":"Genetic Engineering Team, RIKEN Center for Life Science Technologies, Kobe 650-0047, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Aizawa","sequence":"additional","affiliation":[{"name":"Genetic Engineering Team, RIKEN Center for Life Science Technologies, Kobe 650-0047, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Grahammer","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"III. Medizinische Klinik, Universit\u00e4tsklinikum Hamburg-Eppendorf, 20251 Hamburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Hartleben","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rn","family":"Dengjel","sequence":"additional","affiliation":[{"name":"BIOSS Centre for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, 79106 Freiburg, Germany;"},{"name":"Department of Dermatology, Faculty of Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"Center for Biological Systems Analysis (ZBSA) and Freiburg Institute for Advanced Studies, Albert-Ludwigs-University, 79106 Freiburg, Germany;"},{"name":"Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias B.","family":"Huber","sequence":"additional","affiliation":[{"name":"Department of Medicine IV, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany;"},{"name":"BIOSS Centre for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, 79106 Freiburg, Germany;"},{"name":"III. Medizinische Klinik, Universit\u00e4tsklinikum Hamburg-Eppendorf, 20251 Hamburg, Germany;"},{"name":"Center for Biological Systems Analysis (ZBSA) and Freiburg Institute for Advanced Studies, Albert-Ludwigs-University, 79106 Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,5,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.06.006"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.169"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32832e316d"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm403"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014030278"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2014.00160"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2012.291"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3769"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1110813"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.10.047"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.01.022"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005090921"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200603073"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI69778"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.487"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46458"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3257"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.07.016"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008080871"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036705"},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"e01149","DOI":"10.7554\/eLife.01149","article-title":"Nanoscale protein architecture of the kidney glomerular basement membrane","volume":"2","author":"Suleiman H","year":"2013","unstructured":"H Suleiman, , Nanoscale protein architecture of the kidney glomerular basement membrane. eLife 2, e01149 (2013).","journal-title":"eLife"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012080844"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2216"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.10.041"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.27958"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201104118"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.109"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.324"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.5"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.05.004"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2014.00207"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00616.2014"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67846"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.08.025"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08067"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200712086"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.07.001"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2156"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/21541248.2015.1113353"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00730-13"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2011.08.002"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.07.008"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3843"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016010008"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1617004114","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1617004114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:29:57Z","timestamp":1649816997000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1617004114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,23]]},"references-count":45,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2017,6,6]]}},"alternative-id":["10.1073\/pnas.1617004114"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1617004114","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,23]]},"assertion":[{"value":"2017-05-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}