{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:32:18Z","timestamp":1789194738878,"version":"build-2803163510"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"21","license":[{"start":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T00:00:00Z","timestamp":1510272000000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001349","name":"MOH | National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC CBRG11nov105"],"award-info":[{"award-number":["NMRC CBRG11nov105"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012415","name":"Biomedical Research Council","doi-asserted-by":"crossref","award":["BMRC 04\/1\/21\/19\/316"],"award-info":[{"award-number":["BMRC 04\/1\/21\/19\/316"]}],"id":[{"id":"10.13039\/501100012415","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,5,23]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    To directly address in vivo significance of the altered Ca\n                    <jats:sub>V<\/jats:sub>\n                    1.2 channel property arising from alternative splicing, we generated Ca\n                    <jats:sub>V<\/jats:sub>\n                    1.2 exon 33-specific knockout (exon 33\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice. Here, we showed that the exclusion of alternative exon 33 altered Ca\n                    <jats:sub>V<\/jats:sub>\n                    1.2 biophysical property, leading to greater\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>Ca<\/jats:sub>\n                    density. This increase in current density induced prolongation of ventricular cardiomyocyte action potential duration, and the cardiomyocytes exhibited increased early afterdepolarizations and autonomous action potentials\u2014hallmarks of arrhythmias. In vivo, exon 33\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice had increased occurrences of premature ventricular contractions, tachycardia, and lengthened QT interval. As such, exclusion of exon 33 of the Ca\n                    <jats:sub>V<\/jats:sub>\n                    1.2 channel is proarrhythmogenic. Although failing human hearts had greater inclusion of exon 33, it is unclear whether the inclusion is compensatory, neutral, or damaging.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1617205114","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T09:47:53Z","timestamp":1494496073000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":25,"title":["Exclusion of alternative exon 33 of Ca\n                    <sub>V<\/sub>\n                    1.2 calcium channels in heart is proarrhythmogenic"],"prefix":"10.1073","volume":"114","author":[{"given":"Guang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Physiology, National University of Singapore, 117597 Singapore;"},{"name":"Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research, Southwest Medical University, Sichuan, 646000 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Physiology, National University of Singapore, 117597 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tan Fong","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Physiology, National University of Singapore, 117597 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuk Peng","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Physiology, National University of Singapore, 117597 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Physiology, National University of Singapore, 117597 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology, National University of Singapore, 117597 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for chamber quantification: A report from the American Society of Echocardiography\u2019s Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology","volume":"18","author":"Lang RM","year":"2005","unstructured":"RM Lang, , Recommendations for chamber quantification: A report from the American Society of Echocardiography\u2019s Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology. 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