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U.S.A."],"published-print":{"date-parts":[[2017,6,20]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Bcc bacteria are intrinsically resistant to multiple antibiotics. They are major pathogens in patients with cystic fibrosis (CF) and can cause severe necrotizing pneumonia, which is often fatal. Hopanoid biosynthesis is one of the major mechanisms involved in multiple antimicrobial resistance of Bcc pathogens. The\n                    <jats:italic>hpnN<\/jats:italic>\n                    gene of\n                    <jats:italic>B. multivorans<\/jats:italic>\n                    encodes an integral membrane protein of the HpnN family of transporters, which is responsible for shuttling hopanoids to the outer membrane. Here, we report crystal structures of\n                    <jats:italic>B. multivorans<\/jats:italic>\n                    HpnN that indicate a plausible pathway for hopanoid transport. Overall our data suggest a novel mechanism for hopanoid transport involved in cell wall remodeling, which is critical for mediating multidrug resistance in\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.1619660114","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T20:50:17Z","timestamp":1496695817000},"page":"6557-6562","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":57,"title":["Crystal structures of the\n                    <i>Burkholderia multivorans<\/i>\n                    hopanoid transporter HpnN"],"prefix":"10.1073","volume":"114","author":[{"given":"Nitin","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Chemistry, Iowa State University, Ames, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chia","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, Iowa State University, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung-Han","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, Iowa State University, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhijith","family":"Radhakrishnan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Iowa State University, Ames, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared A.","family":"Delmar","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, Iowa State University, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanagalaghatta R.","family":"Rajashankar","sequence":"additional","affiliation":[{"name":"Northeastern Collaborative Access Team, Argonne National Laboratory, Argonne, IL 60439;"},{"name":"Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward W.","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Iowa State University, Ames, IA 50011;"},{"name":"Department of Physics and Astronomy, Iowa State University, IA 50011;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,6,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/nrmicro1085","article-title":"The multifarious, multireplicon Burkholderia cepacia complex","volume":"3","author":"Mahenthiralingam E","year":"2005","unstructured":"E Mahenthiralingam, TA Urban, JB Goldberg, The multifarious, multireplicon Burkholderia cepacia complex. 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Thorax 53, 432\u2013436 (1998).","journal-title":"Thorax"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1513\/AnnalsATS.201408-395OC","article-title":"Burkholderia species infections in patients with cystic fibrosis in British Columbia, Canada. 30 years\u2019 experience","volume":"12","author":"Zlosnik JE","year":"2015","unstructured":"JE Zlosnik, , Burkholderia species infections in patients with cystic fibrosis in British Columbia, Canada. 30 years\u2019 experience. 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Clin Microbiol Infect 16, 821\u2013830 (2010).","journal-title":"Clin Microbiol Infect"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1136\/thx.50.11.1194","article-title":"Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak","volume":"50","author":"Whiteford ML","year":"1995","unstructured":"ML Whiteford, , Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak. 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