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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,5,23]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Methods for studying functional genomics of ion-channel target sites of antinematodal drugs (anthelmintics) have been limited to the model nematode\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    because many techniques for studying animal parasitic nematodes have been unsuccessful. Here, we develop preparations of the human parasite\n                    <jats:italic>Brugia malayi,<\/jats:italic>\n                    which causes elephantiasis, to allow us to combine RNAi, PCR amplification of RNA from single muscle cells, computer analysis of movement, and patch-clamp electrophysiology to study the action of cholinergic anthelmintics. We find that they are not a homogenous class of drugs and that they act selectively on four distinguishable,\n                    <jats:italic>M-<\/jats:italic>\n                    ,\n                    <jats:italic>L-<\/jats:italic>\n                    ,\n                    <jats:italic>P-<\/jats:italic>\n                    , and\n                    <jats:italic>N-<\/jats:italic>\n                    acetylcholine ion-channel receptors. These four receptors are distinct druggable targets with different physiological functions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1619820114","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T20:40:35Z","timestamp":1494362435000},"page":"5539-5544","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":38,"title":["Functional genomics in\n                    <i>Brugia malayi<\/i>\n                    reveal diverse muscle nAChRs and differences between cholinergic anthelmintics"],"prefix":"10.1073","volume":"114","author":[{"given":"Saurabh","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhanva Srinivas","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan Patrick","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard John","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Iowa State University, Ames, IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,5,9]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68653-4"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1425.000"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000149"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000256"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2011.09.002"},{"key":"e_1_3_3_6_2","first-page":"1","article-title":"Nematode genome evolution","author":"Coghlan A","year":"2005","unstructured":"A Coghlan, Nematode genome evolution. 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