{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T02:27:01Z","timestamp":1789871221972,"version":"4.0.1"},"reference-count":59,"publisher":"National Academy of Sciences","issue":"9","license":[{"start":{"date-parts":[[2017,8,13]],"date-time":"2017-08-13T00:00:00Z","timestamp":1502582400000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB0745611"],"award-info":[{"award-number":["MCB0745611"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","award":["DE-SC 0001035"],"award-info":[{"award-number":["DE-SC 0001035"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["2P41GM103422"],"award-info":[{"award-number":["2P41GM103422"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["2P41-GM104601"],"award-info":[{"award-number":["2P41-GM104601"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,2,28]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Photosystem II (PSII) is a multisubunit membrane protein complex that catalyzes light-driven oxidation of water, supplying energy to nearly all life on Earth. Because of the challenging chemistry it performs, PSII undergoes frequent damage, prompting an intricate cycle of disassembly, repair, and reassembly. Psb28, a protein that binds transiently to the RC47 intermediate complex, protects PSII from light-induced damage. The location of Psb28 in PSII has not been determined. In this study, we used chemical cross-linking, mass spectrometry, and protein docking to demonstrate that Psb28 binds to cytochrome\n                    <jats:italic>b<\/jats:italic>\n                    <jats:sub>559<\/jats:sub>\n                    . Our results allow us to propose a mechanism by which Psb28 exerts its protective effect on the vulnerable RC47 complex.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1620360114","type":"journal-article","created":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T21:00:27Z","timestamp":1487019627000},"page":"2224-2229","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":49,"title":["Mass spectrometry-based cross-linking study shows that the Psb28 protein binds to cytochrome\n                    <i>b<\/i>\n                    <sub>559<\/sub>\n                    in Photosystem II"],"prefix":"10.1073","volume":"114","author":[{"given":"Daniel A.","family":"Weisz","sequence":"first","affiliation":[{"name":"Department of Biology, Washington University, St. Louis, MO 63130;"},{"name":"Department of Chemistry, Washington University, St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biology, Washington University, St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington University, St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundarapandian","family":"Thangapandian","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8434-1010","authenticated-orcid":false,"given":"Emad","family":"Tajkhorshid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Gross","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington University, St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himadri B.","family":"Pakrasi","sequence":"additional","affiliation":[{"name":"Department of Biology, Washington University, St. Louis, MO 63130;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,2,13]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13991"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2011.07.006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00617"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2014.09.007"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09913"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.11.007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2015.08.031"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050312-120124"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.01.006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111597108"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00157"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.467613"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.184184"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.246231"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408458200"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi026012"},{"key":"e_1_3_3_17_2","first-page":"297","volume-title":"Proceedings of the Xth International Photosynthesis Congress","author":"Ikeuchi M","year":"1995","unstructured":"M Ikeuchi, Y Inoue, W Vermaas, Characterization of Photosystem II subunits from the cyanobacterium Synechocystis sp. PCC 6803. Photosynthesis: From light to biosphere. Proceedings of the Xth International Photosynthesis Congress, ed P Mathis (Kluwer, Dordrecht), pp. 297\u2013300 (1995)."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2014.02.013"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.130039"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2012.10.004"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0066"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.08.001"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.10.008"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2014.960852"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2010.10.014"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0685-7_30"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.1.109"},{"key":"e_1_3_3_28_2","first-page":"1233","article-title":"A new 4.8-kDa polypeptide intrinsic to the PS II reaction center, as revealed by modified SDS-PAGE with improved resolution of low-molecular-weight proteins","volume":"29","author":"Ikeuchi M","year":"1988","unstructured":"M Ikeuchi, Y Inoue, A new 4.8-kDa polypeptide intrinsic to the PS II reaction center, as revealed by modified SDS-PAGE with improved resolution of low-molecular-weight proteins. Plant Cell Physiol 29, 1233\u20131239 (1988).","journal-title":"Plant Cell Physiol"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1323063111"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00147a027"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.238394"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2012.02.017"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200305"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3617"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9673"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.03.005"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1221483"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2103"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2458"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr301162j"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.014126"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22876"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2011.08.002"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(95)00003-2"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006015214868"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2011.07.012"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1608862113"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1991.tb08753.x"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-0511-5_78"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.01261"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-007-9249-y"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.59"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-008-9350-x"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.123919"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801917200"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-64"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.034009"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000190"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.23304"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1620360114","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1620360114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:09:49Z","timestamp":1649815789000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1620360114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,13]]},"references-count":59,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,2,28]]}},"alternative-id":["10.1073\/pnas.1620360114"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1620360114","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,13]]},"assertion":[{"value":"2017-02-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}