{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:23:33Z","timestamp":1789759413401,"version":"4.0.1"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,8,5]]},"abstract":"<jats:p>\n                    In yeasts and mammals,\n                    <jats:italic>PEX10<\/jats:italic>\n                    encodes an integral membrane protein\n with a C\n                    <jats:sub>3<\/jats:sub>\n                    HC\n                    <jats:sub>4<\/jats:sub>\n                    RING finger motif in its C-terminal domain\n and is required for peroxisome biogenesis and matrix protein import. In\n humans, its dysfunction in peroxisome biogenesis leads to severe Zellweger\n Syndrome and infantile Refsum disease. Here we show that dysfunction of a\n homologous gene in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    leads to lethality at the heart stage\n of embryogenesis, impairing the biogenesis of peroxisomes, lipid bodies, and\n protein bodies. In a T-DNA insertion mutant disrupting the fourth exon of the\n                    <jats:italic>AthPEX10<\/jats:italic>\n                    gene, ultrastructural analyses fail to detect peroxisomes\n characteristic for wild-type embryogenesis. Storage triacyl glycerides are not\n assembled into lipid bodies (oil bodies; oleosomes) surrounded by the\n phospholipid\u2013protein monolayer membrane. Instead, the dysfunctional\n monolayer membranes, which derive from the bilayer membrane of the endoplasmic\n reticulum, accumulate in the cytosol. Concomitantly the transfer of the\n storage proteins from their site of synthesis at the endoplasmic reticulum to\n the vacuoles is disturbed. The mutant can be rescued by transformation with\n wild-type\n                    <jats:italic>AthPEX10<\/jats:italic>\n                    cDNA. Transformants of wild-type\n                    <jats:italic>Hansenula\n polymorpha<\/jats:italic>\n                    cells with the\n                    <jats:italic>AthPEX10<\/jats:italic>\n                    cDNA did produce the encoded\n protein without targeting it to peroxisomes. Additionally, the cDNA could not\n complement a\n                    <jats:italic>Hansenula pex10<\/jats:italic>\n                    mutant unable to form peroxisomes. The\n ultrastructural knockout phenotype of\n                    <jats:italic>Ath<\/jats:italic>\n                    PEX10p suggests that this\n protein in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    is essential for peroxisome, oleosome, and\n protein transport vesicle formation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1633697100","type":"journal-article","created":{"date-parts":[[2003,8,5]],"date-time":"2003-08-05T13:14:48Z","timestamp":1060089288000},"page":"9626-9631","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":89,"title":["AthPEX10, a nuclear gene essential for peroxisome and storage organelle formation during\n                    <i>Arabidopsis<\/i>\n                    embryogenesis"],"prefix":"10.1073","volume":"100","author":[{"given":"Uwe","family":"Schumann","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Botanik, Technische\r Universit\u00e4t M\u00fcnchen, Am Hochanger 4, D-85350 Freising, Germany;\r Department Biologie I,\r Ludwig-Maximilians-Universit\u00e4t, Menzinger Strasse 67, D-80638\r M\u00fcnchen, Germany; and Laboratory for\r Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30,\r 9751 NN Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Wanner","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Botanik, Technische\r Universit\u00e4t M\u00fcnchen, Am Hochanger 4, D-85350 Freising, Germany;\r Department Biologie I,\r Ludwig-Maximilians-Universit\u00e4t, Menzinger Strasse 67, D-80638\r M\u00fcnchen, Germany; and Laboratory for\r Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30,\r 9751 NN Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marten","family":"Veenhuis","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Botanik, Technische\r Universit\u00e4t M\u00fcnchen, Am Hochanger 4, D-85350 Freising, Germany;\r Department Biologie I,\r Ludwig-Maximilians-Universit\u00e4t, Menzinger Strasse 67, D-80638\r M\u00fcnchen, Germany; and Laboratory for\r Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30,\r 9751 NN Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Schmid","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Botanik, Technische\r Universit\u00e4t M\u00fcnchen, Am Hochanger 4, D-85350 Freising, Germany;\r Department Biologie I,\r Ludwig-Maximilians-Universit\u00e4t, Menzinger Strasse 67, D-80638\r M\u00fcnchen, Germany; and Laboratory for\r Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30,\r 9751 NN Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Gietl","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Botanik, Technische\r Universit\u00e4t M\u00fcnchen, Am Hochanger 4, D-85350 Freising, Germany;\r Department Biologie I,\r Ludwig-Maximilians-Universit\u00e4t, Menzinger Strasse 67, D-80638\r M\u00fcnchen, Germany; and Laboratory for\r Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30,\r 9751 NN Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,7,25]]},"reference":[{"key":"e_1_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(00)01865-1"},{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","unstructured":"Charlton W. & Lopez-Huertas E. (2002) in Plant Peroxisomes eds. Baker A. & Graham I. A.\n (Kluwer Academic Dordrecht The Netherlands) pp.\n 385-426.","DOI":"10.1007\/978-94-015-9858-3_12"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(01)01951-3"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.4.761"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.9.1399"},{"key":"e_1_3_1_6_2","first-page":"25700","volume":"275","year":"2000","unstructured":"Okumoto, K., Abe, I. & Fujiki, Y. (2000) J. Cell Biol. 275, 25700-25710.","journal-title":"J. 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