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(2013).","DOI":"10.2210\/pdb4lg9\/pdb"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.tibs.2010.04.003","article-title":"WD40 proteins propel cellular networks","volume":"35","author":"Stirnimann CU","year":"2010","unstructured":"CU Stirnimann, E Petsalaki, RB Russell, CW M\u00fcller, WD40 proteins propel cellular networks. Trends Biochem Sci 35, 565\u2013574 (2010).","journal-title":"Trends Biochem Sci"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"W299","DOI":"10.1093\/nar\/gki370","article-title":"ConSurf 2005: The projection of evolutionary conservation scores of residues on protein structures","volume":"33","author":"Landau M","year":"2005","unstructured":"M Landau, , ConSurf 2005: The projection of evolutionary conservation scores of residues on protein structures. Nucleic Acids Res 33, W299\u2013302 (2005).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/nsmb.2209","article-title":"Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance","volume":"19","author":"Migliori V","year":"2012","unstructured":"V Migliori, , Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance. Nat Struct Mol Biol 19, 136\u2013144 (2012).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"19266","DOI":"10.1073\/pnas.1008937107","article-title":"Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2)","volume":"107","author":"Xu C","year":"2010","unstructured":"C Xu, , Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2). Proc Natl Acad Sci USA 107, 19266\u201319271 (2010).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1002\/humu.10194","article-title":"RettBASE: The IRSA MECP2 variation database-a new mutation database in evolution","volume":"21","author":"Christodoulou J","year":"2003","unstructured":"J Christodoulou, A Grimm, T Maher, B Bennetts, RettBASE: The IRSA MECP2 variation database-a new mutation database in evolution. Hum Mutat 21, 466\u2013472 (2003).","journal-title":"Hum Mutat"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.ajhg.2009.03.010","article-title":"DECIPHER: Database of Chromosomal Imbalance and Phenotype in Humans Using Ensembl Resources","volume":"84","author":"Firth HV","year":"2009","unstructured":"HV Firth, , DECIPHER: Database of Chromosomal Imbalance and Phenotype in Humans Using Ensembl Resources. Am J Hum Genet 84, 524\u2013533 (2009).","journal-title":"Am J Hum Genet"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1126\/science.1227764","article-title":"Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders","volume":"338","author":"O\u2019Roak BJ","year":"2012","unstructured":"BJ O\u2019Roak, , Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders. 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