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Here we demonstrated the sequential process of T4P dynamics from stimulus to taxis at the single-cell level in a model cyanobacterium, which can recognize light direction. We directly visualized that T4P filaments dominantly appeared from the side of the cell opposite the illumination. This asymmetric activation is regulated on a timescale of minutes, and the process was transitioned between three sequential phases. These findings provide clues toward a general regulation mechanism of the T4P system.<\/jats:p>","DOI":"10.1073\/pnas.1702395114","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T20:50:17Z","timestamp":1496695817000},"page":"6593-6598","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":70,"title":["Asymmetric distribution of type IV pili triggered by directional light in unicellular cyanobacteria"],"prefix":"10.1073","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8201-2608","authenticated-orcid":false,"given":"Daisuke","family":"Nakane","sequence":"first","affiliation":[{"name":"Department of Physics, Gakushuin University, Tokyo 171-8588, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Nishizaka","sequence":"additional","affiliation":[{"name":"Department of Physics, Gakushuin University, Tokyo 171-8588, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,6,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2762"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.121171698"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35024105"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad2001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911328107"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402305101"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242523299"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.148"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00023"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12486"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.04.007"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pce010"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02068.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1999.tb01954.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.5.1599-1607.2003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131201098"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvi089"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.12620"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024974111818"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.51.1.1-21.1987"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161737"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/370104a0"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5076"},{"key":"e_1_3_3_24_2","first-page":"700","article-title":"Appendages of the cyanobacterial cell","volume":"5","author":"Schuergers N","year":"2015","unstructured":"N Schuergers, A Wilde, Appendages of the cyanobacterial cell. Life (Basel) 5, 700\u2013715 (2015).","journal-title":"Life (Basel)"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000064"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4759"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.104.178104"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1525538113"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja301540r"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802149105"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.01.042"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060087"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1502025112"},{"key":"e_1_3_3_34_2","first-page":"458","article-title":"Genetics of the blue light-dependent signal cascade that controls phototaxis in the cyanobacterium Synechocystis sp. 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