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Two prior crystal structures of OaPAC in the dark state, and mutagenesis experiments, indicate that the activation mechanism involves only very small movements, but we have now succeeded in refining the structure of the light-activated protein to high resolution, showing in molecular detail the changes at the chromophore on light exposure and allowing precise comparison of the structure in the light-exposed and dark states. The differences between these structures indicate the very small but concerted shifts that trigger enzyme activity tens of \u00e5ngstroms from the chromophore.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1704391114","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T16:52:26Z","timestamp":1501692746000},"page":"8562-8567","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":46,"title":["Molecular mechanism of photoactivation of a light-regulated adenylate cyclase"],"prefix":"10.1073","volume":"114","author":[{"given":"Mio","family":"Ohki","sequence":"first","affiliation":[{"name":"Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, Tsurumi, Yokohama 230-0045, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayana","family":"Sato-Tomita","sequence":"additional","affiliation":[{"name":"Division of Biophysics, Department of Physiology, Jichi Medical University, Shimotsuke, Tochigi 329-0498, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics KK, Hamakita-ku, Hamamatsu, Shizuoka 434-8601, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mineo","family":"Iseki","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy R. 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