{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:05:07Z","timestamp":1792587907581,"version":"4.5.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"37","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["24780293"],"award-info":[{"award-number":["24780293"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["25304040"],"award-info":[{"award-number":["25304040"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["26660220"],"award-info":[{"award-number":["26660220"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["15J00686"],"award-info":[{"award-number":["15J00686"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["15K19069"],"award-info":[{"award-number":["15K19069"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16J00854"],"award-info":[{"award-number":["16J00854"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16K15032"],"award-info":[{"award-number":["16K15032"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["50421988"],"award-info":[{"award-number":["50421988"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007697","name":"Northern Advancement Center for Science and Technology","doi-asserted-by":"publisher","award":["H25 T-3-13"],"award-info":[{"award-number":["H25 T-3-13"]}],"id":[{"id":"10.13039\/501100007697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,9,12]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Flaviviruses represent a significant threat to public health worldwide, and several cause severe neurological disease in humans and animals. However, no specific treatment has been developed, due to the lack of information about their detailed pathogenic mechanisms. In the current study, we reveal that the transport of viral RNA of tick-borne flavivirus in neuronal dendrites is involved in the development of neurological disease. The virus hijacked the transport system of host mRNA in dendrites, which is important for neuronal functions such as neurogenesis and the plasticity of the synaptic communication. Our finding of this unique virus\u2013host interaction will promote the study of neurodegenerative diseases caused by disruption of dendritic mRNA transport and the development of their treatment.<\/jats:p>","DOI":"10.1073\/pnas.1704454114","type":"journal-article","created":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T21:00:52Z","timestamp":1503954052000},"page":"9960-9965","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":36,"title":["Dendritic transport of tick-borne flavivirus RNA by neuronal granules affects development of neurological disease"],"prefix":"10.1073","volume":"114","author":[{"given":"Minato","family":"Hirano","sequence":"first","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Memi","family":"Muto","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuki","family":"Sakai","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirofumi","family":"Kondo","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Kariwa","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Yoshii","sequence":"additional","affiliation":[{"name":"Laboratory of Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,8,28]]},"reference":[{"key":"e_1_3_3_1_2","unstructured":"BD Lindenbach HJ Thiel CM Rice Flaviviridae : The viruses and their replication. Fields Virology eds Knipe DM Howley PM (Lippincott-Raven Philadelphia) 5th Ed pp 1101\u20131152. (2007)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01283-14"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17636"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60238-X"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760150192"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2210.160818"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0000000000000166"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2010.03278.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/342059"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.061432-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.278"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01844-07"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6386-09.2010"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010564107"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep20775"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701532104"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep10745"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.8.4099-4113.2006"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.05.039"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11737"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2395"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7092861"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02225-08"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01342-07"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1444206"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/80591"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.76"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902833106"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.04.003"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2008.07.007"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2009.09.013"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab3369"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-10-26"},{"key":"e_1_3_3_34_2","first-page":"e2373","article-title":"Nucleofection and primary culture of embryonic mouse hippocampal and cortical neurons","author":"Viesselmann C","year":"2011","unstructured":"C Viesselmann, J Ballweg, D Lumbard, EW Dent, Nucleofection and primary culture of embryonic mouse hippocampal and cortical neurons. J Vis Exp, pp. e2373 (2011).","journal-title":"J Vis Exp"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1943-1947.1997"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-011-1066-0"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2016.11.029"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80169-0"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_3_3_40_2","first-page":"e1787","article-title":"A simple composite phenotype scoring system for evaluating mouse models of cerebellar ataxia","author":"Guyenet SJ","year":"2010","unstructured":"SJ Guyenet, , A simple composite phenotype scoring system for evaluating mouse models of cerebellar ataxia. J Vis Exp, pp. e1787 (2010).","journal-title":"J Vis Exp"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1704454114","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1704454114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:50:51Z","timestamp":1649818251000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1704454114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,28]]},"references-count":41,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2017,9,12]]}},"alternative-id":["10.1073\/pnas.1704454114"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1704454114","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,28]]},"assertion":[{"value":"2017-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}