{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:22:42Z","timestamp":1790490162007,"version":"4.1.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"29","license":[{"start":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T00:00:00Z","timestamp":1515110400000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI097206"],"award-info":[{"award-number":["1R01AI097206"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,7,18]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Aside from its undisputed role in the import of newly synthesized proteins into the endoplasmic reticulum (ER), the Sec61 translocon was proposed to ensure the reverse transport of misfolded proteins to the cytosol. Based on this model, Sec61 was also proposed to be the channel exporting internalized antigens from endosomes to the cytosol, for degradation and cross-presentation. Establishing Sec61\u2019s contribution to these connected trafficking pathways has nevertheless proven difficult, due to a technical incapacity to blunt its activity acutely. Here, we took advantage of a recently identified Sec61 blocker to determine whether or not Sec61 can mediate retrograde protein transport. Both ER-to-cytosol and endosome-to-cytosol protein export were intact in mycolactone-treated cells, which argues against Sec61 operating as a retrotranslocon.<\/jats:p>","DOI":"10.1073\/pnas.1705242114","type":"journal-article","created":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T21:00:21Z","timestamp":1499288421000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export"],"prefix":"10.1073","volume":"114","author":[{"given":"Jeff E.","family":"Grotzke","sequence":"first","affiliation":[{"name":"Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrycja","family":"Kozik","sequence":"additional","affiliation":[{"name":"Centre de Recherche, Institut Curie, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-David","family":"Morel","sequence":"additional","affiliation":[{"name":"Immunobiology of Infection Unit, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM, U1221, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Impens","sequence":"additional","affiliation":[{"name":"Vlaams Instituut voor Biotechnologie (VIB)-UGent Center for Medical Biotechnology, 9000 Ghent, Belgium;"},{"name":"VIB Proteomics Core, 9000 Ghent, Belgium;"},{"name":"Department of Biochemistry, Ghent University, 9000 Ghent, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Pietrosemoli","sequence":"additional","affiliation":[{"name":"Center of Bioinformatics, Biostatistics, and Integrative Biology, Institut Pasteur, Unit\u00e9 de Service et de Recherche 3756 Institut Pasteur CNRS, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Cresswell","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520;"},{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Amigorena","sequence":"additional","affiliation":[{"name":"Centre de Recherche, Institut Curie, 75005 Paris, France;"},{"name":"INSERM, U932, 75005 Paris, France;"},{"name":"CBT507 Institut Gustave Roussy-Curie, INSERM Center of Clinical Investigation, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Demangel","sequence":"additional","affiliation":[{"name":"Immunobiology of Infection Unit, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM, U1221, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,7,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-13-0102"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3254"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12432"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.05.002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2546"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-14-56"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117260"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.07.050"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.08.017"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2010.01.022"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh184"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.04.008"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20160662"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5403.854"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1242\/jcs.182352","article-title":"Mechanistic insights into the inhibition of Sec61-dependent co- and post-translational translocation by mycolactone","volume":"129","author":"McKenna M","year":"2016","unstructured":"M McKenna, RE Simmonds, S High, Mechanistic insights into the inhibition of Sec61-dependent co- and post-translational translocation by mycolactone. 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