{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:05:47Z","timestamp":1791295547335,"version":"4.1.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"37","license":[{"start":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T00:00:00Z","timestamp":1503878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,9,12]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Invasion of human intestinal epithelial cells resulting from the delivery of bacterial effectors into the host cell cytoplasm via a type III secretion system (T3SS) is the hallmark of\n                    <jats:italic>Shigella<\/jats:italic>\n                    , the causal agent of bacillary dysentery. Here, we provide evidence that human lymphocytes are mainly targeted by injection of T3SS effectors not resulting in cell invasion. These findings highlight the diversity of mechanisms triggered by\n                    <jats:italic>Shigella<\/jats:italic>\n                    to enlarge the panel of targeted cells and thus counteract host immunity, including adaptive immune responses. The potential impact of these data on vaccine design, in particular for live-attenuated vaccine candidates, deserves further investigation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1707098114","type":"journal-article","created":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T21:00:52Z","timestamp":1503954052000},"page":"9954-9959","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Injection of T3SS effectors not resulting in invasion is the main targeting mechanism of\n                    <i>Shigella<\/i>\n                    toward human lymphocytes"],"prefix":"10.1073","volume":"114","author":[{"given":"Laurie","family":"Pinaud","sequence":"first","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatoumata","family":"Samassa","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziv","family":"Porat","sequence":"additional","affiliation":[{"name":"Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 7610001, Israel;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana L.","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilia","family":"Belotserkovsky","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Parsot","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"},{"name":"Microbiologie et Maladies Infectieuses, Coll\u00e8ge de France, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois-Xavier","family":"Campbell-Valois","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armelle","family":"Phalipon","sequence":"additional","affiliation":[{"name":"Molecular Microbial Pathogenesis Unit, Department of Cellular Biology of Infection, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,8,28]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb7100025"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.07.016"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.12"},{"key":"e_1_3_3_5_2","first-page":"231","article-title":"Shigella infection of intestinal epithelium and circumvention of the host innate defense system","volume":"337","author":"Ashida H","year":"2009","unstructured":"H Ashida, M Ogawa, H Mimuro, C Sasakawa, Shigella infection of intestinal epithelium and circumvention of the host innate defense system. Curr Top Microbiol Immunol 337, 231\u2013255 (2009).","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.03.010"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300981110"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130914"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.16.5486-5495.2004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.11.007"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5347.84"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06863.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04645.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.323"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.1.283"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.10.003"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.08.006"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.04.022"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2016.04.002"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.20.6.795"},{"key":"e_1_3_3_22_2","first-page":"93","article-title":"Surface morphology of mitogen-activated human lymphocytes and their derivatives in vitro","volume":"140","author":"Cuschieri A","year":"1985","unstructured":"A Cuschieri, S Mughal, Surface morphology of mitogen-activated human lymphocytes and their derivatives in vitro. J Anat 140, 93\u2013104 (1985).","journal-title":"J Anat"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.11.2681-2688.1987"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000551"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030196"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041120"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.7.4244"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4A0615-252R"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.2.461-469.1986"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.002"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00028"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00219"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00939-10"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071698"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.852-860.1982"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.18.5899-5906.1993"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.1.124-129.1985"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.01.005"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00856-1"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.4161\/auto.7.9.16252"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1707098114","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1707098114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:50:08Z","timestamp":1649818208000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1707098114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,28]]},"references-count":41,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2017,9,12]]}},"alternative-id":["10.1073\/pnas.1707098114"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1707098114","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,28]]},"assertion":[{"value":"2017-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}