{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:02:58Z","timestamp":1793145778268,"version":"4.5.0"},"reference-count":20,"publisher":"National Academy of Sciences","issue":"35","license":[{"start":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T00:00:00Z","timestamp":1517961600000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI081759"],"award-info":[{"award-number":["AI081759"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD075665"],"award-info":[{"award-number":["HD075665"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","award":["PATH"],"award-info":[{"award-number":["PATH"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI049820"],"award-info":[{"award-number":["AI049820"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,8,29]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Little is known about the specific mechanisms by which viruses associated with congenital disease, such as Zika virus (ZIKV), breach the placental barrier to access the fetal compartment. This is at least in part because of the lack of appropriate systems to model the unique properties of human placental syncytiotrophoblasts, which form a key cellular interface between the maternal and fetal compartments. Here, we describe the development of new organotypic models of human syncytiotrophoblasts that can be used to study the innate defense mechanisms used by these cells to restrict ZIKV infection at various stages of human gestation. These models can be used to study the mechanisms of ZIKV vertical transmission, the development of ZIKV diagnostics, and the testing of anti-ZIKV therapeutics.<\/jats:p>","DOI":"10.1073\/pnas.1707513114","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T10:10:52Z","timestamp":1502187052000},"page":"9433-9438","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":115,"title":["Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal\u2013fetal interface"],"prefix":"10.1073","volume":"114","author":[{"given":"Jacqueline","family":"Corry","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213;"},{"name":"Center for Microbial Pathogenesis, Children\u2019s Hospital of Pittsburgh of the University of Pittsburgh Medical Center, Pittsburgh, PA 15224;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitin","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213;"},{"name":"Center for Microbial Pathogenesis, Children\u2019s Hospital of Pittsburgh of the University of Pittsburgh Medical Center, Pittsburgh, PA 15224;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Good","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213;"},{"name":"Center for Microbial Pathogenesis, Children\u2019s Hospital of Pittsburgh of the University of Pittsburgh Medical Center, Pittsburgh, PA 15224;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2969-6737","authenticated-orcid":false,"given":"Yoel","family":"Sadovsky","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213;"},{"name":"Department of Obstetrics, Gynecology, and Reproductive Science, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn B.","family":"Coyne","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213;"},{"name":"Center for Microbial Pathogenesis, Children\u2019s Hospital of Pittsburgh of the University of Pittsburgh Medical Center, Pittsburgh, PA 15224;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,8,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.chom.2017.04.007","article-title":"Microbial vertical transmission during human pregnancy","volume":"21","author":"Arora N","year":"2017","unstructured":"N Arora, Y Sadovsky, TS Dermody, CB Coyne, Microbial vertical transmission during human pregnancy. Cell Host Microbe 21, 561\u2013567 (2017).","journal-title":"Cell Host Microbe"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"71.e1","DOI":"10.1016\/j.ajog.2014.07.060","article-title":"Human trophoblasts confer resistance to viruses implicated in perinatal infection","volume":"212","author":"Bayer A","year":"2015","unstructured":"A Bayer, , Human trophoblasts confer resistance to viruses implicated in perinatal infection. Am J Obstet Gynecol 212, 71.e1\u201371.e8 (2015).","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.chom.2016.03.008","article-title":"Type III interferons produced by human placental trophoblasts confer protection against Zika virus infection","volume":"19","author":"Bayer A","year":"2016","unstructured":"A Bayer, , Type III interferons produced by human placental trophoblasts confer protection against Zika virus infection. Cell Host Microbe 19, 705\u2013712 (2016).","journal-title":"Cell Host Microbe"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"12048","DOI":"10.1073\/pnas.1304718110","article-title":"Human placental trophoblasts confer viral resistance to recipient cells","volume":"110","author":"Delorme-Axford E","year":"2013","unstructured":"E Delorme-Axford, , Human placental trophoblasts confer viral resistance to recipient cells. Proc Natl Acad Sci USA 110, 12048\u201312053 (2013).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.placenta.2016.09.008","article-title":"Isolation of human trophoblastic extracellular vesicles and characterization of their cargo and antiviral activity","volume":"47","author":"Ouyang Y","year":"2016","unstructured":"Y Ouyang, , Isolation of human trophoblastic extracellular vesicles and characterization of their cargo and antiviral activity. Placenta 47, 86\u201395 (2016).","journal-title":"Placenta"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/nrmicro.2016.125","article-title":"Zika virus\u2014Reigniting the TORCH","volume":"14","author":"Coyne CB","year":"2016","unstructured":"CB Coyne, HM Lazear, Zika virus\u2014Reigniting the TORCH. Nat Rev Microbiol 14, 707\u2013715 (2016).","journal-title":"Nat Rev Microbiol"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1172\/JCI41211","article-title":"The placenta: Transcriptional, epigenetic, and physiological integration during development","volume":"120","author":"Maltepe E","year":"2010","unstructured":"E Maltepe, AI Bakardjiev, SJ Fisher, The placenta: Transcriptional, epigenetic, and physiological integration during development. 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