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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2017,10,17]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Mutations in genes encoding sarcomeric proteins are the major cause of primary inherited cardiomyopathies. Troponin T (TnT), encoded by\n                    <jats:italic>TNNT2<\/jats:italic>\n                    , harbors most of its pathogenic mutants at TNT1 (residues \u223c80\u2013180 of TnT). TNT1 is known to interact with tropomyosin (Tm). In this study, we have analyzed TNT1 mutants using in vitro and in silico methods and correlated the results. We also found a striking correlation between binding affinities for Tm and changes in the calcium sensitivity of regulated actomyosin ATPase activities within residues 92\u2013144. These data are consistent with reducing or increasing the affinity of TnT for Tm as the primary cause of cardiomyopathy for mutations in this region, suggesting a smaller Tm binding region.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1710354114","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T20:40:29Z","timestamp":1506976829000},"page":"11115-11120","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":38,"title":["Molecular mechanisms and structural features of cardiomyopathy-causing troponin T mutants in the tropomyosin overlap region"],"prefix":"10.1073","volume":"114","author":[{"given":"Binnu","family":"Gangadharan","sequence":"first","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"},{"name":"Manipal University, Manipal 576104, India;"},{"name":"National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret S.","family":"Sunitha","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souhrid","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu Roy","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Manauli 140306, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Haque","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"},{"name":"National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendrakumar","family":"Sekar","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramanathan","family":"Sowdhamini","sequence":"additional","affiliation":[{"name":"National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Spudich","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"},{"name":"Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Mercer","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India;"},{"name":"McLaughlin Research Institute, Great Falls, MT 59405"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2017,10,2]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.amjcard.2004.03.007","article-title":"Prevalence of hypertrophic cardiomyopathy in a population-based sample of American Indians aged 51 to 77 years (the Strong Heart Study)","volume":"93","author":"Maron BJ","year":"2004","unstructured":"BJ Maron, , Prevalence of hypertrophic cardiomyopathy in a population-based sample of American Indians aged 51 to 77 years (the Strong Heart Study). 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