{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:15:56Z","timestamp":1793049356328,"version":"4.5.0"},"reference-count":70,"publisher":"National Academy of Sciences","issue":"14","license":[{"start":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T00:00:00Z","timestamp":1521417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2018,4,3]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Bacterial viruses (bacteriophages) append a variety of molecules, including sugars, amino acids, and polyamines, to the nucleobases of their genomic DNA to circumvent the endonuclease-based defenses of their hosts. These DNA hypermodifications are formed through bacteriophage-encoded biosynthetic pathways, with steps occurring before and after replication of bacteriophage DNA. We report here the discovery of two thymidine hypermodifications: 5-(2-aminoethoxy)methyluridine replacing 40% of thymidine nucleotides in the\n                    <jats:italic>Salmonella<\/jats:italic>\n                    phage ViI and 5-(2-aminoethyl)uridine replacing 30% of thymidine in the DNA of the\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    phage M6. Additionally, we show in vitro reconstitution of 5-(2-aminoethyl)uridine biosynthesis from five recombinantly expressed proteins. These findings reveal an expanded diversity in the types of naturally occurring DNA modifications and their biosynthetic pathways.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1714812115","type":"journal-article","created":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T15:19:21Z","timestamp":1521472761000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses"],"prefix":"10.1073","volume":"115","author":[{"given":"Yan-Jiun","family":"Lee","sequence":"first","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Dai","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon E.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan E.","family":"Fraser","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn M.","family":"Kauffman","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chudi","family":"Guan","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3169-6878","authenticated-orcid":false,"suffix":"Jr.","given":"Ivan R.","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter R.","family":"Weigele","sequence":"additional","affiliation":[{"name":"Research Department, New England Biolabs, Ipswich, MA 01938;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,3,19]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00114"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/270369a0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.25.2.500-509.1978"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68724-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.34.2.347-353.1980"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01642396"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.39.2.536-547.1981"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00515a043"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt573"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1195"},{"key":"e_1_3_3_12_2","unstructured":"AMB Kropinski Isolation and characterization of a bacteriophage active against pseudomonas acidovorans. 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