{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:08:58Z","timestamp":1790698138564,"version":"4.1.0"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"27","license":[{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"vor","delay-in-days":183,"URL":"http:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute","doi-asserted-by":"publisher","award":["CA202177"],"award-info":[{"award-number":["CA202177"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2018,7,3]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Systemic inflammation that occurs during the course of tumor treatment is often correlated with adverse oncological outcomes independent of the infectious insult. As the first line of defense against infection, it is likely that neutrophils play an important role in modulating the progression of distant metastases in these scenarios. This study uses a multiplexed microfluidic model of human microcirculation combined with LPS-stimulated neutrophils as a model of systemic infection to probe the dynamic interactions between intravascular tumor cells and neutrophils at high spatiotemporal resolutions. We uncover chemokine-dependent neutrophil migration patterns which result in enhanced tumor cell extravasation rates. These findings may provide a basis for inhibiting the proextravasation and possibly prometastatic effect of inflamed neutrophils.<\/jats:p>","DOI":"10.1073\/pnas.1715932115","type":"journal-article","created":{"date-parts":[[2018,6,18]],"date-time":"2018-06-18T16:44:51Z","timestamp":1529340291000},"page":"7022-7027","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":183,"title":["Inflamed neutrophils sequestered at entrapped tumor cells via chemotactic confinement promote tumor cell extravasation"],"prefix":"10.1073","volume":"115","author":[{"given":"Michelle B.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Hajal","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Benjamin","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139;"},{"name":"Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesham","family":"Azizgolshani","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard O.","family":"Hynes","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139;"},{"name":"Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger D.","family":"Kamm","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"},{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;"},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD 20815"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,6,18]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0329"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3024"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.52"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4048\/jbc.2013.16.1.55"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16140"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14282"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1411082111"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4442"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2393"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.08.012"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/701067"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-011-1721-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060886"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200510-1556OC"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3182862e7e"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-012-2643-5"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2007.06.038"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67484"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S60114"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1591"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200212000-00009"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-2072"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3ib40149a"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1325"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-15-1157"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.018"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.2.641"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-202747"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000494"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.249"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1715932115","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1715932115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T02:39:40Z","timestamp":1649817580000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1715932115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,18]]},"references-count":30,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2018,7,3]]}},"alternative-id":["10.1073\/pnas.1715932115"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1715932115","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,18]]},"assertion":[{"value":"2018-06-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}