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Here, we present a reporter-based technology to identify in vivo repressed (\n                    <jats:italic>ivr<\/jats:italic>\n                    ) genes of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    . Our data demonstrate that constitutive expression of\n                    <jats:italic>ivr<\/jats:italic>\n                    genes reduces colonization fitness, indicating a pivotal role of gene silencing. A comprehensive characterization of an H\n                    <jats:sup>+<\/jats:sup>\n                    \/Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    transporter revealed a defined spatiotemporal expression pattern, being induced in the stomach to facilitate survival under low pH, but silenced under alkaline pH in the lower gastrointestinal tract to avoid detrimental effects. Thus, previously understudied\n                    <jats:italic>ivr<\/jats:italic>\n                    genes could reveal new insights into host\u2013pathogen interaction and alternative therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1716973115","type":"journal-article","created":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T14:35:34Z","timestamp":1519137334000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":34,"title":["In vivo repressed genes of\n                    <i>Vibrio cholerae<\/i>\n                    reveal inverse requirements of an H\n                    <sup>+<\/sup>\n                    \/Cl\n                    <sup>\u2212<\/sup>\n                    transporter along the gastrointestinal passage"],"prefix":"10.1073","volume":"115","author":[{"given":"Fatih","family":"Cakar","sequence":"first","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz G.","family":"Zingl","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Moisi","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Reidl","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria;"},{"name":"BioTechMed Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7842-0177","authenticated-orcid":false,"given":"Stefan","family":"Schild","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria;"},{"name":"BioTechMed Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,2,20]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.VMBF-0015-2015"},{"key":"e_1_3_3_2_2","unstructured":"; World Health Organization Cholera Situation in Yemen November 2017 . 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