{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:28:01Z","timestamp":1787005681552,"version":"build-2736575974"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"5","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI128862"],"award-info":[{"award-number":["AI128862"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI087849"],"award-info":[{"award-number":["AI087849"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2018,1,30]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Enzymes that activate dioxygen typically rely on flavins or transition metals as redox cofactors. We describe the discovery of the enzyme catalyst Cap15 that converts 5\u2032-C-glycyluridine (GlyU), an unusual \u03b2\u2013hydroxy-\u03b1-amino acid, to the modified nucleoside uridine-5\u2032-carboxamide. In contrast to expectations, pyridoxal-5'-phosphate (PLP) is the sole cofactor that serves as the initiating reducing agent to activate dioxygen for incorporation into GlyU prior to decarboxylation. Thus, Cap15 is now classified as an O\n                    <jats:sub>2<\/jats:sub>\n                    - and PLP-dependent monooxygenase-decarboxylase. Also in contrast to expectations, phosphate and the (5\u2032\n                    <jats:italic>S<\/jats:italic>\n                    ,6\u2032\n                    <jats:italic>R<\/jats:italic>\n                    ) isomer of GlyU\u2014and not (5\u2032\n                    <jats:italic>S<\/jats:italic>\n                    ,6\u2032\n                    <jats:italic>S<\/jats:italic>\n                    )-GlyU, the established pathway intermediate\u2014are essential for Cap15 activity. In total, Cap15 utilizes an enzymatic strategy for oxygen incorporation during conversion of an \u03b1-amino acid to a carboxamide.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1718667115","type":"journal-article","created":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T15:13:07Z","timestamp":1516201987000},"page":"974-979","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":31,"title":["Pyridoxal-5\u2032-phosphate as an oxygenase cofactor: Discovery of a carboxamide-forming, \u03b1-amino acid monooxygenase-decarboxylase"],"prefix":"10.1073","volume":"115","author":[{"given":"Ying","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536;"},{"name":"Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Wiegmann","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmaceutical and Medicinal Chemistry, Saarland University, 66123 Saarbr\u00fccken, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Niro","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmaceutical and Medicinal Chemistry, Saarland University, 66123 Saarbr\u00fccken, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Ducho","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmaceutical and Medicinal Chemistry, Saarland University, 66123 Saarbr\u00fccken, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, People\u2019s Republic of China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyong","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven G.","family":"Van Lanen","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,1,17]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-273"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2011.05.019"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2009"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b01016"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.56.243"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.57.639"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2007.88"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.39.1047"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2009.38"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.393"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.646414"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12554"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229521"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja308185q"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1318117"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2010.03.037"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201404775"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(71)18345-0"},{"key":"e_1_3_3_19_2","first-page":"37","volume-title":"Transaminases","author":"Kallen RG","year":"1985","unstructured":"RG Kallen, , Chemical and spectroscopic properties of pyridoxal and pyridoxamine phosphates. 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