{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:57:21Z","timestamp":1781107041746,"version":"3.54.1"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,10,29]]},"abstract":"<jats:p>\n                    Recent findings indicate that glycosaminoglycans can play important roles in animal development. The genes\n                    <jats:italic>sqv<\/jats:italic>\n                    -\n                    <jats:italic>3<\/jats:italic>\n                    , -\n                    <jats:italic>7<\/jats:italic>\n                    , and -\n                    <jats:italic>8<\/jats:italic>\n                    , which are necessary for vulval morphogenesis in\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    , affect the biosynthesis of chondroitin and heparan sulfate glycosaminoglycans. We cloned\n                    <jats:italic>sqv<\/jats:italic>\n                    -\n                    <jats:italic>1<\/jats:italic>\n                    and showed that the SQV-1 protein is a type II transmembrane protein that functions as a UDP-glucuronic acid decarboxylase. SQV-1 localizes to punctate cytoplasmic compartments and colocalizes with the SQV-7 nucleotide-sugar transporter, which probably acts in the Golgi apparatus. SQV-1 and SQV-7 are both expressed in the vulva and in oocytes, where they likely act in vulval morphogenesis and embryonic development, respectively. Progeny of\n                    <jats:italic>sqv<\/jats:italic>\n                    -\n                    <jats:italic>7<\/jats:italic>\n                    and\n                    <jats:italic>sqv<\/jats:italic>\n                    -\n                    <jats:italic>1<\/jats:italic>\n                    null mutants fail to initiate cytokinesis, possibly because they are unable to separate the plasma membrane from the eggshell, a defect analogous to that of incomplete vulval invagination.\n                  <\/jats:p>","DOI":"10.1073\/pnas.172522199","type":"journal-article","created":{"date-parts":[[2002,10,29]],"date-time":"2002-10-29T12:24:11Z","timestamp":1035894251000},"page":"14218-14223","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":73,"title":["The SQV-1 UDP-glucuronic acid decarboxylase and the SQV-7 nucleotide-sugar transporter may act in the Golgi apparatus to affect\n                    <i>Caenorhabditis elegans<\/i>\n                    vulval morphogenesis and embryonic development"],"prefix":"10.1073","volume":"99","author":[{"given":"Ho-Yon","family":"Hwang","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, 68-425, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Robert","family":"Horvitz","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, 68-425, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,10,21]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.13.2623"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.16.3055"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.18.3565"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/22343"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/27932"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90630-U"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.3.819"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9798"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.293.5535.1670"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.3.968"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.3.974"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.002303"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.22915"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26165"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061593098"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172522499"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/77.1.71"},{"key":"e_1_3_3_18_2","unstructured":"Riddle D. 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