{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:46:45Z","timestamp":1790362005621,"version":"4.1.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,8,19]]},"abstract":"<jats:p>\n                    Cul1, a member of the cullin ubiquitin ligase family, forms a multiprotein\n complex known as SCF and plays an essential role in numerous cellular and\n biological activities. A Cul1 homologue, p185 (Cul7), has been isolated as an\n simian virus 40 large T antigen-binding protein. To understand the\n physiological role of p185, we generated mice lacking p185.\n                    <jats:italic>p185<\/jats:italic>\n                    <jats:sup>\u2013<\/jats:sup>\n                    <jats:sup>\/<\/jats:sup>\n                    <jats:sup>\u2013<\/jats:sup>\n                    embryos are\n runted and die immediately after birth because of respiratory distress. Dermal\n and hypodermal hemorrhage is detected in mutant embryos at late gestational\n stage.\n                    <jats:italic>p185<\/jats:italic>\n                    <jats:sup>\u2013<\/jats:sup>\n                    <jats:sup>\/<\/jats:sup>\n                    <jats:sup>\u2013<\/jats:sup>\n                    placentas\n show defects in the differentiation of the trophoblast lineage with an\n abnormal vascular structure. We demonstrate that p185 forms an SCF-like\n complex with Skp1, Rbx1, Fbw6 (Fbx29), and FAP68 (FAP48, glomulin).\n                    <jats:italic>FAP68<\/jats:italic>\n                    has recently been identified as a gene responsible for familial\n glomuvenous malformation. These results suggest that p185 forms a multiprotein\n complex and plays an important role in vascular morphogenesis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1733908100","type":"journal-article","created":{"date-parts":[[2003,8,19]],"date-time":"2003-08-19T12:56:21Z","timestamp":1061297781000},"page":"9855-9860","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":128,"title":["Targeted disruption of\n                    <i>p185\/Cul7<\/i>\n                    gene results in abnormal vascular morphogenesis"],"prefix":"10.1073","volume":"100","author":[{"given":"Takehiro","family":"Arai","sequence":"first","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn S.","family":"Kasper","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey R.","family":"Skaar","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Hamid","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"DeCaprio","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana\u2013Farber Cancer Institute, 44\r Binney Street, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,8,6]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.425"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.15.1.435"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90271-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80753-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251541198"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00890"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81267-2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/13886"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80024-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.18.2375"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.14.4997-5005.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80021-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80020-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80098-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80403-1"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(98)01473-5"},{"key":"e_1_3_2_18_2","first-page":"435","volume":"9","year":"1998","unstructured":"Michel, J. 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