{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:25:46Z","timestamp":1793121946035,"version":"4.5.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"26","license":[{"start":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T00:00:00Z","timestamp":1544486400000},"content-version":"vor","delay-in-days":183,"URL":"http:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2016YFA0501500"],"award-info":[{"award-number":["2016YFA0501500"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81490740"],"award-info":[{"award-number":["81490740"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21472008"],"award-info":[{"award-number":["21472008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21521003"],"award-info":[{"award-number":["21521003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2018,6,26]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Baicalin is a major flavonoid component from the herbal medicine\n                    <jats:italic>Scutellaria baicalensis<\/jats:italic>\n                    that has been shown to have an antisteatosis effect. Through quantitative chemoproteomic profiling, we discovered that baicalin acts as a natural allosteric activator of carnitine palmitoyltransferase 1 (CPT1), the rate-limiting enzyme of fatty acid \u03b2-oxidation (FAO). By directly binding to CPT1 and activating its activity to accelerate fatty acid degradation, baicalin can significantly ameliorate symptoms associated with hepatic steatosis and reduce diet-induced obesity (DIO). Our study provides an example of a natural product agonist for CPT1. The results provide mechanistic insights to explain the bioactivity of baicalin in reducing lipid accumulation and introduce exciting opportunities for developing novel flavonoid-based FAO activators for pharmacologically treating DIO and associated metabolic disorders.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1801745115","type":"journal-article","created":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T15:26:40Z","timestamp":1528730800000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":302,"title":["Chemoproteomics reveals baicalin activates hepatic CPT1 to ameliorate diet-induced obesity and hepatic steatosis"],"prefix":"10.1073","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2865-8587","authenticated-orcid":false,"given":"Jianye","family":"Dai","sequence":"first","affiliation":[{"name":"Synthetic and Functional Biomolecules Center, Peking University, 100871 Beijing, China;"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Liang","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"School of Life Sciences, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentong","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China;"},{"name":"University of the Chinese Academy of Sciences, 101408 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Synthetic and Functional Biomolecules Center, Peking University, 100871 Beijing, China;"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongkun","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Lv","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"Institute of Molecular Medicine, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Engineering, Peking University, 100871 Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Engineering, Peking University, 100871 Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shentian","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Synthetic and Functional Biomolecules Center, Peking University, 100871 Beijing, China;"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Hou","sequence":"additional","affiliation":[{"name":"Synthetic and Functional Biomolecules Center, Peking University, 100871 Beijing, China;"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiannian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Engineering, Peking University, 100871 Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"Institute of Molecular Medicine, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyi","family":"Huang","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"College of Engineering, Peking University, 100871 Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Ping","family":"Xiao","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"Institute of Molecular Medicine, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ling","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2156-3198","authenticated-orcid":false,"given":"Tuoping","family":"Luo","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1822-1701","authenticated-orcid":false,"given":"Junyu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"},{"name":"School of Life Sciences, Peking University, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6925-1268","authenticated-orcid":false,"given":"Chu","family":"Wang","sequence":"additional","affiliation":[{"name":"Synthetic and Functional Biomolecules Center, Peking University, 100871 Beijing, China;"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, China;"},{"name":"Peking-Tsinghua Center for Life Sciences, 100871 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,6,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.181"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.183"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.041"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2014.44"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.28595"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.008"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.07.003"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.011"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.03.009"},{"key":"e_1_3_3_10_2","volume-title":"Biochemistry","author":"Berg JM","year":"2002","unstructured":"JM Berg, JL Tymoczko, L Stryer, L Stryer Biochemistry (Freeman, 5th Ed, New York, 2002).","edition":"5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00001.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2004.06.004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2009.00642.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27300"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2011.10.008"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132128899"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507300102"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.11.020"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi052186q"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-005-0576-5"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/74.4.418"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0631"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2337\/db10-0589"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M032631"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.11.026"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17040569"},{"key":"e_1_3_3_27_2","first-page":"698","article-title":"[Progress in pharmacologic research on Scutellaria baicalensis]","volume":"9","author":"Li Z","year":"1989","unstructured":"Z Li, XY Guo, [Progress in pharmacologic research on Scutellaria baicalensis]. Zhong Xi Yi Jie He Za Zhi 9, 698\u2013700. Chinese (1989).","journal-title":"Zhong Xi Yi Jie He Za Zhi"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/food.200300382"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2009.150"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1159\/000374037"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b00862"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060713-035708"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep07896"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1769"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2368"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5CC10445A"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.138"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1413706111"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.062906.171838"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.11.049"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2005.07.021"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-1468"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304481200"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00497.2007"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b11575"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.01.022"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1801745115","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1801745115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T03:33:04Z","timestamp":1649820784000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1801745115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,11]]},"references-count":47,"journal-issue":{"issue":"26","published-print":{"date-parts":[[2018,6,26]]}},"alternative-id":["10.1073\/pnas.1801745115"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1801745115","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,11]]},"assertion":[{"value":"2018-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}