{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:38:33Z","timestamp":1792636713700,"version":"4.5.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"40","license":[{"start":{"date-parts":[[2018,8,27]],"date-time":"2018-08-27T00:00:00Z","timestamp":1535328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2018,10,2]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Parental health and diet at the time of conception determine the development and life-long disease risk of their offspring. While the association between poor maternal diet and offspring health is well established, the underlying mechanisms linking paternal diet with offspring health are poorly defined. Possible programming pathways include changes in testicular and sperm epigenetic regulation and status, seminal plasma composition, and maternal reproductive tract responses regulating early embryo development. In this study, we demonstrate that paternal low-protein diet induces sperm-DNA hypomethylation in conjunction with blunted female reproductive tract embryotrophic, immunological, and vascular remodeling responses. Furthermore, we identify sperm- and seminal plasma-specific programming effects of paternal diet with elevated offspring adiposity, metabolic dysfunction, and altered gut microbiota.<\/jats:p>","DOI":"10.1073\/pnas.1806333115","type":"journal-article","created":{"date-parts":[[2018,8,27]],"date-time":"2018-08-27T19:45:44Z","timestamp":1535399144000},"page":"10064-10069","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":273,"title":["Paternal diet programs offspring health through sperm- and seminal plasma-specific pathways in mice"],"prefix":"10.1073","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0842-1251","authenticated-orcid":false,"given":"Adam J.","family":"Watkins","sequence":"first","affiliation":[{"name":"Aston Research Centre for Healthy Ageing, School of Life and Health Sciences, Aston University, B4 7ET Birmingham, United Kingdom;"},{"name":"Division of Child Health, Obstetrics and Gynaecology, Faculty of Medicine, University of Nottingham, NG7 2UH Nottingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irundika","family":"Dias","sequence":"additional","affiliation":[{"name":"Aston Research Institute, Aston Medical School, Aston University, B4 7ET Birmingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Tsuro","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences, Aston University, B4 7ET Birmingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Allen","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences, Aston University, B4 7ET Birmingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Emes","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, United Kingdom;"},{"name":"Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Moreton","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, United Kingdom;"},{"name":"Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray","family":"Wilson","sequence":"additional","affiliation":[{"name":"Deep Seq, Centre for Genetics and Genomics, University of Nottingham, Queen\u2019s Medical Centre, NG7 2UH Nottingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8021-2705","authenticated-orcid":false,"given":"Richard J. M.","family":"Ingram","sequence":"additional","affiliation":[{"name":"Nottingham Digestive Diseases Centre, Queens Medical Centre, Nottingham University Hospitals National Health Service Trust, NG7 2UH Nottingham, United Kingdom;"},{"name":"Centre for Biomedical Sciences, University of Nottingham, NG7 2RD Nottingham, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6375-215X","authenticated-orcid":false,"given":"Kevin D.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,8,27]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30312-X"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-62414-3_8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2009.01032.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.07.012"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep29787"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01092.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2010.09.038"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09491"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.12.008"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00981.2013"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.02.009"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der192"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.06.047"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09899-9"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad7977"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3889"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gam028"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102736"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305609111"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/1933719113493511"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2017.07.013"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.6.1382"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002695"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-1340"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-7-104"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.121699"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002440"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2013.08.001"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab2006"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12107"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2011.631627"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.08.016"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107890"},{"key":"e_1_3_3_34_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1165\/rcmb.2012-0525OC","article-title":"Pivotal role of the 5-lipoxygenase pathway in lung injury after experimental sepsis","volume":"50","author":"Monteiro AP","year":"2014","unstructured":"AP Monteiro, , Pivotal role of the 5-lipoxygenase pathway in lung injury after experimental sepsis. Am J Respir Cell Mol Biol 50, 87\u201395 (2014).","journal-title":"Am J Respir Cell Mol Biol"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.26"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2016.01.013"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2013.220"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11695-010-0353-2"},{"key":"e_1_3_3_39_2","first-page":"507","article-title":"SREBP-1c, regulated by the insulin and AMPK signaling pathways, plays a role in nonalcoholic fatty liver disease","volume":"21","author":"Kohjima M","year":"2008","unstructured":"M Kohjima, , SREBP-1c, regulated by the insulin and AMPK signaling pathways, plays a role in nonalcoholic fatty liver disease. Int J Mol Med 21, 507\u2013511 (2008).","journal-title":"Int J Mol Med"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.10.004"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu7010017"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00015"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000001422"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD16119"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610317104"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0187"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep18193"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1806333115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T08:23:06Z","timestamp":1661847786000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1806333115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,27]]},"references-count":47,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2018,10,2]]}},"alternative-id":["10.1073\/pnas.1806333115"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1806333115","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,27]]},"assertion":[{"value":"2018-08-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}