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Here we devised an approach to address the selectivity of the barrier using nitrogen-15\u2013labeled serum proteins as tracers. Following systemic injection of the labeled serum to normal nitrogen-14 mice, the labeled proteins subsequently entered the aqueous fluid and were measured by mass spectrometry. This new quantitative method captured the dynamic redistribution patterns of approximately 500 serum proteins entering normal eye and the eye recovering from injury. We discovered inhibitory complement proteins crossed the blood-ocular barrier of the wounded eye but not of the normal eye.<\/jats:p>","DOI":"10.1073\/pnas.1807982115","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T15:15:33Z","timestamp":1534778133000},"page":"9032-9037","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":16,"title":["Selective permeability of mouse blood-aqueous barrier as determined by\n                    <sup>15<\/sup>\n                    N-heavy isotope tracing and mass spectrometry"],"prefix":"10.1073","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3066-652X","authenticated-orcid":false,"given":"Pan","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Nephrology and Hypertension, Feinberg Cardiovascular and Renal Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin R.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Feinberg Cardiovascular and Renal Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Khalatyan","sequence":"additional","affiliation":[{"name":"Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey N.","family":"Savas","sequence":"additional","affiliation":[{"name":"Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan E.","family":"Quaggin","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Feinberg Cardiovascular and Renal Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7023-5305","authenticated-orcid":false,"given":"Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Feinberg Cardiovascular and Renal Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2018,8,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.5301\/EJO.2010.6049","article-title":"Blood-retinal barrier","volume":"21","author":"Cunha-Vaz J","year":"2011","unstructured":"J Cunha-Vaz, R Bernardes, C Lobo, Blood-retinal barrier. 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