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To identify cryptococcal antigens that could serve as vaccine candidates by stimulating T cell responses,\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    -reactive CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell hybridomas were generated by immunization of C57BL\/6 mice and fusion of splenocytes with thymoma cells. The antigen that stimulated one of the hybridomas, designated P1D6, to produce IL-2 was purified to homogeneity by sequential anion exchange chromatography, hydrophobic interaction chromatography, and SDS\/PAGE. Based on its apparent molecular mass of 98 kDa and mannosylation, the antigen of interest was named MP98. MP98 was N terminal-sequenced, and the gene encoding the protein was cloned and sequenced. Recombinant MP98, expressed in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    , stimulated P1D6 to produce IL-2. Analysis of the derived 458-aa sequence of MP98 reveals an N-terminal cleavable signal sequence, a polysaccharide deacetylase domain found in fungal chitin deacetylases, and a serine\/threonine-rich C-terminal region. Overall, there were 103 serine\/threonine residues serving as potential O-linked glycosylation sites as well as 12 possible N-linked glycosylation sites. Thus, a\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    mannoprotein has been characterized that stimulates T cell responses and has molecular properties of a chitin deacetylase.\n                  <\/jats:p>","DOI":"10.1073\/pnas.181331398","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"10422-10427","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":131,"title":["Molecular characterization of a mannoprotein with homology to chitin deacetylases that stimulates T cell responses to\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1073","volume":"98","author":[{"given":"Stuart M.","family":"Levitz","sequence":"first","affiliation":[{"name":"Evans Memorial Department of Clinical Research and Department of Medicine, Boston University School of Medicine, Boston, MA 02118; and Goldman School of Dental Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-hua","family":"Nong","sequence":"additional","affiliation":[{"name":"Evans Memorial Department of Clinical Research and Department of Medicine, Boston University School of Medicine, Boston, MA 02118; and Goldman School of Dental Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Mansour","sequence":"additional","affiliation":[{"name":"Evans Memorial Department of Clinical Research and Department of Medicine, Boston University School of Medicine, Boston, MA 02118; and Goldman School of Dental Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Huang","sequence":"additional","affiliation":[{"name":"Evans Memorial Department of Clinical Research and Department of Medicine, Boston University School of Medicine, Boston, MA 02118; and Goldman School of Dental Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Specht","sequence":"additional","affiliation":[{"name":"Evans Memorial Department of Clinical Research and Department of Medicine, Boston University School of Medicine, Boston, MA 02118; and Goldman School of Dental Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,8,14]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1093\/clinids\/13.6.1163","volume":"13","author":"Levitz S M","year":"1991","unstructured":"S M Levitz Rev Infect Dis 13, 1163\u20131169 (1991).","journal-title":"Rev Infect Dis"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","DOI":"10.1128\/9781555818241","volume-title":"Cryptococcus neoformans","author":"Casadevall A","year":"1998","unstructured":"A Casadevall, J R Perfect Cryptococcus neoformans (Am. 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