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Stem Cells Dev 22, 473\u2013482 (2013).","journal-title":"Stem Cells Dev"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1038\/ncomms6619","article-title":"An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator","volume":"5","author":"Lee DS","year":"2014","unstructured":"DS Lee, , An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator. Nat Commun 5, 5619 (2014).","journal-title":"Nat Commun"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/nature10716","article-title":"DNA-binding factors shape the mouse methylome at distal regulatory regions","volume":"480","author":"Stadler MB","year":"2011","unstructured":"MB Stadler, , DNA-binding factors shape the mouse methylome at distal regulatory regions. 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Mol Biol Evol 34, 1702\u20131712 (2017).","journal-title":"Mol Biol Evol"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1101\/gr.164913.113","article-title":"Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mechanism of establishment","volume":"24","author":"Court F","year":"2014","unstructured":"F Court, , Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mechanism of establishment. Genome Res 24, 554\u2013569 (2014).","journal-title":"Genome Res"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1038\/nature13544","article-title":"The DNA methylation landscape of human early embryos","volume":"511","author":"Guo H","year":"2014","unstructured":"H Guo, , The DNA methylation landscape of human early embryos. Nature 511, 606\u2013610 (2014).","journal-title":"Nature"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"19483","DOI":"10.1074\/jbc.M110.131995","article-title":"Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells","volume":"285","author":"Liu L","year":"2010","unstructured":"L Liu, , Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. J Biol Chem 285, 19483\u201319490 (2010).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature02402","article-title":"Birth of parthenogenetic mice that can develop to adulthood","volume":"428","author":"Kono T","year":"2004","unstructured":"T Kono, , Birth of parthenogenetic mice that can develop to adulthood. Nature 428, 860\u2013864 (2004).","journal-title":"Nature"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/nature23286","article-title":"Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation","volume":"548","author":"Yagi M","year":"2017","unstructured":"M Yagi, , Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation. Nature 548, 224\u2013227 (2017).","journal-title":"Nature"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1126\/science.1061402","article-title":"Epigenetic instability in ES cells and cloned mice","volume":"293","author":"Humpherys D","year":"2001","unstructured":"D Humpherys, , Epigenetic instability in ES cells and cloned mice. Science 293, 95\u201397 (2001).","journal-title":"Science"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nature09017","article-title":"Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells","volume":"465","author":"Stadtfeld M","year":"2010","unstructured":"M Stadtfeld, , Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature 465, 175\u2013181 (2010).","journal-title":"Nature"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.cell.2011.12.035","article-title":"Base-resolution analyses of sequence and parent-of-origin dependent DNA methylation in the mouse genome","volume":"148","author":"Xie W","year":"2012","unstructured":"W Xie, , Base-resolution analyses of sequence and parent-of-origin dependent DNA methylation in the mouse genome. 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Biol Reprod 81, 906\u2013920 (2009).","journal-title":"Biol Reprod"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"e135","DOI":"10.1371\/journal.pbio.0060135","article-title":"The evolution of the DLK1-DIO3 imprinted domain in mammals","volume":"6","author":"Edwards CA","year":"2008","unstructured":"CA Edwards, , The evolution of the DLK1-DIO3 imprinted domain in mammals. PLoS Biol; SAVOIR consortium 6, e135 (2008).","journal-title":"PLoS Biol"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/ng.2007.51","article-title":"Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta","volume":"40","author":"Sekita Y","year":"2008","unstructured":"Y Sekita, , Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta. 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Genes Cells 22, 174\u2013188 (2017).","journal-title":"Genes Cells"},{"key":"e_1_3_4_47_2","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/apha.12160","article-title":"Perturbations to the IGF1 growth pathway and adult energy homeostasis following disruption of mouse chromosome 12 imprinting","volume":"210","author":"Charalambous M","year":"2014","unstructured":"M Charalambous, ST da Rocha, A Hernandez, AC Ferguson-Smith, Perturbations to the IGF1 growth pathway and adult energy homeostasis following disruption of mouse chromosome 12 imprinting. Acta Physiol (Oxf) 210, 174\u2013187 (2014).","journal-title":"Acta Physiol (Oxf)"},{"key":"e_1_3_4_48_2","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.stemcr.2017.12.015","article-title":"XIST derepression in active X chromosome hinders pig somatic cell nuclear transfer","volume":"10","author":"Ruan D","year":"2018","unstructured":"D Ruan, , XIST derepression in active X chromosome hinders pig somatic cell nuclear transfer. Stem Cell Reports 10, 494\u2013508 (2018).","journal-title":"Stem Cell Reports"},{"key":"e_1_3_4_49_2","doi-asserted-by":"crossref","first-page":"14492","DOI":"10.1073\/pnas.1520945114","article-title":"Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium","volume":"113","author":"Biase FH","year":"2016","unstructured":"FH Biase, , Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium. Proc Natl Acad Sci USA 113, 14492\u201314501 (2016).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_50_2","doi-asserted-by":"crossref","first-page":"38439","DOI":"10.1038\/srep38439","article-title":"RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery","volume":"6","author":"S\u00f5ber S","year":"2016","unstructured":"S S\u00f5ber, , RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery. Sci Rep 6, 38439 (2016).","journal-title":"Sci Rep"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","first-page":"42336","DOI":"10.1038\/srep42336","article-title":"Factors associated with aberrant imprint methylation and oligozoospermia","volume":"7","author":"Kobayashi N","year":"2017","unstructured":"N Kobayashi, , Factors associated with aberrant imprint methylation and oligozoospermia. Sci Rep 7, 42336 (2017).","journal-title":"Sci Rep"},{"key":"e_1_3_4_52_2","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.1093\/hmg\/ddm187","article-title":"Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients","volume":"16","author":"Kobayashi H","year":"2007","unstructured":"H Kobayashi, , Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet 16, 2542\u20132551 (2007).","journal-title":"Hum Mol Genet"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/cr.2013.173","article-title":"High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells","volume":"24","author":"Chang G","year":"2014","unstructured":"G Chang, , High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells. Cell Res 24, 293\u2013306 (2014).","journal-title":"Cell Res"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","first-page":"11974","DOI":"10.1073\/pnas.1708710114","article-title":"Rat embryonic stem cells produce fertile offspring through tetraploid complementation","volume":"114","author":"Li TD","year":"2017","unstructured":"TD Li, , Rat embryonic stem cells produce fertile offspring through tetraploid complementation. Proc Natl Acad Sci USA 114, 11974\u201311979 (2017).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_55_2","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.stemcr.2016.02.005","article-title":"Naive pluripotent stem cells derived directly from isolated cells of the human inner cell mass","volume":"6","author":"Guo G","year":"2016","unstructured":"G Guo, , Naive pluripotent stem cells derived directly from isolated cells of the human inner cell mass. Stem Cell Reports 6, 437\u2013446 (2016).","journal-title":"Stem Cell Reports"},{"key":"e_1_3_4_56_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/ng.2007.56","article-title":"Deletions and epimutations affecting the human 14q32.2 imprinted region in individuals with paternal and maternal upd(14)-like phenotypes","volume":"40","author":"Kagami M","year":"2008","unstructured":"M Kagami, , Deletions and epimutations affecting the human 14q32.2 imprinted region in individuals with paternal and maternal upd(14)-like phenotypes. Nat Genet 40, 237\u2013242 (2008).","journal-title":"Nat Genet"},{"key":"e_1_3_4_57_2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3109\/08830189709116851","article-title":"Immunobiology of human melanoma antigens MART-1 and gp100 and their use for immuno-gene therapy","volume":"14","author":"Kawakami Y","year":"1997","unstructured":"Y Kawakami, SA Rosenberg, Immunobiology of human melanoma antigens MART-1 and gp100 and their use for immuno-gene therapy. Int Rev Immunol 14, 173\u2013192 (1997).","journal-title":"Int Rev Immunol"},{"key":"e_1_3_4_58_2","doi-asserted-by":"crossref","first-page":"e1003441","DOI":"10.1371\/journal.pgen.1003441","article-title":"Identification of rtl1, a retrotransposon-derived imprinted gene, as a novel driver of hepatocarcinogenesis","volume":"9","author":"Riordan JD","year":"2013","unstructured":"JD Riordan, , Identification of rtl1, a retrotransposon-derived imprinted gene, as a novel driver of hepatocarcinogenesis. PLoS Genet 9, e1003441 (2013).","journal-title":"PLoS Genet"},{"key":"e_1_3_4_59_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.stem.2018.06.008","article-title":"Loss of H3K27me3 imprinting in somatic cell nuclear transfer embryos disrupts post-implantation development","volume":"23","author":"Matoba S","year":"2018","unstructured":"S Matoba, , Loss of H3K27me3 imprinting in somatic cell nuclear transfer embryos disrupts post-implantation development. Cell Stem Cell 23, 343\u2013354.e5 (2018).","journal-title":"Cell Stem Cell"},{"key":"e_1_3_4_60_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/1471-2164-12-204","article-title":"Recent acquisition of imprinting at the rodent Sfmbt2 locus correlates with insertion of a large block of miRNAs","volume":"12","author":"Wang Q","year":"2011","unstructured":"Q Wang, , Recent acquisition of imprinting at the rodent Sfmbt2 locus correlates with insertion of a large block of miRNAs. BMC Genomics 12, 204 (2011).","journal-title":"BMC Genomics"},{"key":"e_1_3_4_61_2","doi-asserted-by":"crossref","first-page":"e15947","DOI":"10.1371\/journal.pone.0015947","article-title":"Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors","volume":"6","author":"Li Y","year":"2011","unstructured":"Y Li, M Cang, AS Lee, K Zhang, D Liu, Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors. PLoS One 6, e15947 (2011).","journal-title":"PLoS One"},{"key":"e_1_3_4_62_2","first-page":"57","article-title":"A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under tem cell potency fluctuates with endogenous retrovirus activity","volume":"487","author":"Huang B","year":"2011","unstructured":"B Huang, , A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under tem cell potency fluctuates with endogenous retrovirus activity. Nature 487, 57\u201363 (2011).","journal-title":"Nature"},{"key":"e_1_3_4_63_2","doi-asserted-by":"crossref","first-page":"262","DOI":"10.3389\/fmicb.2012.00262","article-title":"The role of genes domesticated from LTR retrotransposons and retroviruses in mammals","volume":"3","author":"Kaneko-Ishino T","year":"2012","unstructured":"T Kaneko-Ishino, F Ishino, The role of genes domesticated from LTR retrotransposons and retroviruses in mammals. Front Microbiol 3, 262 (2012).","journal-title":"Front Microbiol"},{"key":"e_1_3_4_64_2","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.cell.2017.12.022","article-title":"Retrovirus-like gag protein Arc1 binds RNA and traffics across synaptic boutons","volume":"172","author":"Ashley J","year":"2018","unstructured":"J Ashley, , Retrovirus-like gag protein Arc1 binds RNA and traffics across synaptic boutons. Cell 172, 262\u2013274.e11 (2018).","journal-title":"Cell"},{"key":"e_1_3_4_65_2","first-page":"e24501","article-title":"The neuronal gene Arc encodes a repurposed retrotransposon gag protein that chemically defined conditions of dual kinase inhibition","volume":"6","author":"Pastuzyn ED","year":"2018","unstructured":"ED Pastuzyn, , The neuronal gene Arc encodes a repurposed retrotransposon gag protein that chemically defined conditions of dual kinase inhibition. PLoS One 6, e24501 (2018).","journal-title":"PLoS One"},{"key":"e_1_3_4_66_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature11244","article-title":"Embryonic stem cell potency fluctuates with endogenous retrovirus activity","volume":"487","author":"Macfarlan TS","year":"2012","unstructured":"TS Macfarlan, , Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487, 57\u201363 (2012).","journal-title":"Nature"},{"key":"e_1_3_4_67_2","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1073\/pnas.1615735114","article-title":"piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice","volume":"114","author":"Xu C","year":"2017","unstructured":"C Xu, , piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice. Proc Natl Acad Sci USA 114, 722\u2013727 (2017).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_68_2","doi-asserted-by":"crossref","first-page":"8662","DOI":"10.1038\/s41598-017-09061-z","article-title":"Convergent and divergent genetic changes in the genome of Chinese and European pigs","volume":"7","author":"Wang J","year":"2017","unstructured":"J Wang, , Convergent and divergent genetic changes in the genome of Chinese and European pigs. Sci Rep 7, 8662 (2017).","journal-title":"Sci Rep"},{"key":"e_1_3_4_69_2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead B","year":"2012","unstructured":"B Langmead, SL Salzberg, Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357\u2013359 (2012).","journal-title":"Nat Methods"},{"key":"e_1_3_4_70_2","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1101\/gr.107524.110","article-title":"The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data","volume":"20","author":"McKenna A","year":"2010","unstructured":"A McKenna, , The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res 20, 1297\u20131303 (2010).","journal-title":"Genome Res"},{"key":"e_1_3_4_71_2","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1038\/ng.2443","article-title":"Epigenomic analysis detects widespread gene-body DNA hypomethylation in chronic lymphocytic leukemia","volume":"44","author":"Kulis M","year":"2012","unstructured":"M Kulis, , Epigenomic analysis detects widespread gene-body DNA hypomethylation in chronic lymphocytic leukemia. Nat Genet 44, 1236\u20131242 (2012).","journal-title":"Nat Genet"},{"key":"e_1_3_4_72_2","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1093\/bioinformatics\/btr167","article-title":"Bismark: A flexible aligner and methylation caller for Bisulfite-seq applications","volume":"27","author":"Krueger F","year":"2011","unstructured":"F Krueger, SR Andrews, Bismark: A flexible aligner and methylation caller for Bisulfite-seq applications. Bioinformatics 27, 1571\u20131572 (2011).","journal-title":"Bioinformatics"},{"key":"e_1_3_4_73_2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim D","year":"2015","unstructured":"D Kim, B Langmead, SL Salzberg, HISAT: A fast spliced aligner with low memory requirements. Nat Methods 12, 357\u2013360 (2015).","journal-title":"Nat Methods"},{"key":"e_1_3_4_74_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/1471-2105-15-215","article-title":"Using beta-binomial regression for high-precision differential methylation analysis in multifactor whole-genome bisulfite sequencing experiments","volume":"15","author":"Dolzhenko E","year":"2014","unstructured":"E Dolzhenko, AD Smith, Using beta-binomial regression for high-precision differential methylation analysis in multifactor whole-genome bisulfite sequencing experiments. BMC Bioinformatics 15, 215 (2014).","journal-title":"BMC Bioinformatics"},{"key":"e_1_3_4_75_2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2014A Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders S","year":"2015","unstructured":"S Anders, PT Pyl, W Huber, HTSeq\u2014A Python framework to work with high-throughput sequencing data. Bioinformatics 31, 166\u2013169 (2015).","journal-title":"Bioinformatics"},{"key":"e_1_3_4_76_2","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love MI","year":"2014","unstructured":"MI Love, W Huber, S Anders, Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15, 550 (2014).","journal-title":"Genome Biol"},{"key":"e_1_3_4_77_2","doi-asserted-by":"crossref","first-page":"D764","DOI":"10.1093\/nar\/gkt1168","article-title":"The UCSC genome browser database: 2014 update","volume":"42","author":"Karolchik D","year":"2014","unstructured":"D Karolchik, , The UCSC genome browser database: 2014 update. Nucleic Acids Res 42, D764\u2013D770 (2014).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_78_2","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/nbt.1621","article-title":"Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation","volume":"28","author":"Trapnell C","year":"2010","unstructured":"C Trapnell, , Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol 28, 511\u2013515 (2010).","journal-title":"Nat Biotechnol"},{"key":"e_1_3_4_79_2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1186\/1471-2105-15-311","article-title":"PLEK: A tool for predicting long non-coding RNAs and messenger RNAs based on an improved k-mer scheme","volume":"15","author":"Li A","year":"2014","unstructured":"A Li, J Zhang, Z Zhou, PLEK: A tool for predicting long non-coding RNAs and messenger RNAs based on an improved k-mer scheme. BMC Bioinformatics 15, 311 (2014).","journal-title":"BMC Bioinformatics"},{"key":"e_1_3_4_80_2","doi-asserted-by":"crossref","first-page":"D279","DOI":"10.1093\/nar\/gkv1344","article-title":"The Pfam protein families database: Towards a more sustainable future","volume":"44","author":"Finn RD","year":"2016","unstructured":"RD Finn, , The Pfam protein families database: Towards a more sustainable future. 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