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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,1,29]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Human cytomegalovirus (HCMV) is a widespread pathogen that remains with an individual for life in a quiescent\/latent state, posing little threat to an otherwise healthy person. However, when an individual\u2019s immune system is severely compromised, HCMV can reactivate to its active\/lytic state, resulting in viral spread and disease that is often fatal. The biological mechanisms underlying HCMV latency and reactivation remain poorly understood. Herein we show that the viral-encoded G protein-coupled receptor (GPCR)\n                    <jats:italic>US28<\/jats:italic>\n                    aids in the establishment and the maintenance of viral latency. Furthermore, we find that US28 modulates host-cell proteins to suppress viral processes associated with active\/lytic replication, thereby promoting latent infection. This work provides the mechanism by which HCMV modulates the host-cell environment to its advantage.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1816933116","type":"journal-article","created":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T16:35:23Z","timestamp":1547570123000},"page":"1755-1764","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":75,"title":["Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos"],"prefix":"10.1073","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0919-2961","authenticated-orcid":false,"given":"Benjamin A.","family":"Krishna","sequence":"first","affiliation":[{"name":"Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3252-7670","authenticated-orcid":false,"given":"Monica S.","family":"Humby","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, University at Buffalo, The State University of New York, Buffalo, NY 14203;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6900-4884","authenticated-orcid":false,"given":"William E.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4943-3774","authenticated-orcid":false,"given":"Christine M.","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106;"},{"name":"Department of Microbiology &amp; Immunology, University at Buffalo, The State University of New York, Buffalo, NY 14203;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,1,15]]},"reference":[{"key":"e_1_3_3_1_2","volume-title":"Cytomegaloviruses and Their Replication","author":"Mocarski ES","year":"2001","unstructured":"ES Mocarski, CT Courcelle Cytomegaloviruses and Their Replication (Lippincott Williams & Wilkins, 4th Ed, Philadelphia, 2001).","edition":"4"},{"key":"e_1_3_3_2_2","volume-title":"Cytomegaloviruses","author":"Pass RF","year":"2001","unstructured":"RF Pass Cytomegaloviruses (Lippincott Williams & Wilkins, 4th Ed, Philadelphia, 2001).","edition":"4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.11.006"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/421999"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4437"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw668"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3947\/ic.2013.45.3.260"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2010.03074.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02607-6"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81891-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014509107"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.25.11879"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.6.4017-4021.1994"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00305-15"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252630899"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.1.170.bloodjournal801170"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1556\/AMicr.52.2005.3-4.11"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01166-13"},{"key":"e_1_3_3_19_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1016-8478(23)13505-9","article-title":"Factors affecting human cytomegalovirus gene expression in human monocyte cell lines","volume":"9","author":"Lee CH","year":"1999","unstructured":"CH Lee, , Factors affecting human cytomegalovirus gene expression in human monocyte cell lines. 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