{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:25:05Z","timestamp":1790760305775,"version":"4.1.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"29","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K17108"],"award-info":[{"award-number":["17K17108"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26253065"],"award-info":[{"award-number":["26253065"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["ZIABC011561-05"],"award-info":[{"award-number":["ZIABC011561-05"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ngapore NRF Senior Investigatorship","award":["NRFI2017-02"],"award-info":[{"award-number":["NRFI2017-02"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,7,16]]},"abstract":"<jats:p>\n                    Conventional dendritic cells (cDCs) derive from bone marrow (BM) precursors that undergo cascades of developmental programs to terminally differentiate in peripheral tissues. Pre-cDC1s and pre-cDC2s commit in the BM to each differentiate into CD8\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \/CD103\n                    <jats:sup>+<\/jats:sup>\n                    cDC1s and CD11b\n                    <jats:sup>+<\/jats:sup>\n                    cDC2s, respectively. Although both cDCs rely on the cytokine FLT3L during development, mechanisms that ensure cDC accessibility to FLT3L have yet to be elucidated. Here, we generated mice that lacked a disintegrin and metalloproteinase (ADAM) 10 in DCs (\n                    <jats:italic>Itgax<\/jats:italic>\n                    -cre \u00d7\n                    <jats:italic>Adam10<\/jats:italic>\n                    -fl\/fl; ADAM10\n                    <jats:sup>\u2206DC<\/jats:sup>\n                    ) and found that ADAM10 deletion markedly impacted splenic cDC2 development. Pre-cDC2s accumulated in the spleen with transcriptomic alterations that reflected their inability to differentiate and exhibited abrupt failure to survive as terminally differentiated cDC2s. Induced ADAM10 ablation also led to the reduction of terminally differentiated cDC2s, and restoration of Notch signaling, a major pathway downstream of ADAM10, only modestly rescued them. ADAM10\n                    <jats:sup>\u2206DC<\/jats:sup>\n                    BM failed to generate cDC2s in BM chimeric mice with or without cotransferred ADAM10-sufficient BM, indicating that cDC2 development required cell-autonomous ADAM10. We determined cDC2s to be sources of soluble FLT3L, as supported by decreased serum FLT3L concentration and the retention of membrane-bound FLT3L on cDC2 surfaces in ADAM10\n                    <jats:sup>\u2206DC<\/jats:sup>\n                    mice, and by demonstrating the release of soluble FLT3L by cDC2 in ex vivo culture supernatants. Through in vitro studies utilizing murine embryonic fibroblasts, we determined FLT3L to be a substrate for ADAM10. These data collectively reveal cDC2s as FLT3L sources and highlight a cell-autonomous mechanism that may enhance FLT3L accessibility for cDC2 development and survival.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1818907116","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T20:25:36Z","timestamp":1562012736000},"page":"14714-14723","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Cell-autonomous FLT3L shedding via ADAM10 mediates conventional dendritic cell development in mouse spleen"],"prefix":"10.1073","volume":"116","author":[{"given":"Kohei","family":"Fujita","sequence":"first","affiliation":[{"name":"Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan;"},{"name":"Department of Dermatology, Keio University School of Medicine, Tokyo 160-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetoslav","family":"Chakarov","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, 138648 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Voisin","sequence":"additional","affiliation":[{"name":"Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaibo","family":"Duan","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, 138648 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taneaki","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, National Defense Medical College, Tokorozawa 359-8513, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3314-7052","authenticated-orcid":false,"given":"Masayuki","family":"Amagai","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Keio University School of Medicine, Tokyo 160-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Ginhoux","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, 138648 Singapore;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7005-3138","authenticated-orcid":false,"given":"Keisuke","family":"Nagao","sequence":"additional","affiliation":[{"name":"Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1749-6632.2003.tb06029.x","article-title":"Dendritic cell function in vivo during the steady state: A role in peripheral tolerance","volume":"987","author":"Steinman R. 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