{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:30:48Z","timestamp":1793745048379,"version":"4.6.1"},"reference-count":17,"publisher":"National Academy of Sciences","issue":"13","license":[{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"name":"State Key Projects Specialized for Infectious Diseases of China","award":["2017ZX10201201-006"],"award-info":[{"award-number":["2017ZX10201201-006"]}]},{"name":"National Key R&D program of China","award":["2018YFC1312100"],"award-info":[{"award-number":["2018YFC1312100"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,3,26]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>We detected cfDNA somatic mutations in combination with protein markers to efficiently identify early stage HCC from asymptomatic HBsAg-seropositive individuals in a community population. We designed an assay to profile multiple types of genetic variations in parallel so that 2 mL of plasma were sufficient to achieve high sensitivity in the detection of cfDNA mutations. The assay successfully identified four early-stage HCC cases (&lt;3 cm) from 331 HBsAg (+) individuals who were negative based on screening with serum AFP and ultrasonography. The positive predictive value was 17%, which was much higher than in previous studies. This paper shows that the combination of cfDNA and serum protein markers has significant promise for the early detection of HCC in the community population.<\/jats:p>","DOI":"10.1073\/pnas.1819799116","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T20:18:57Z","timestamp":1552335537000},"page":"6308-6312","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":162,"title":["Detection of early-stage hepatocellular carcinoma in asymptomatic HBsAg-seropositive individuals by liquid biopsy"],"prefix":"10.1073","volume":"116","author":[{"given":"Chunfeng","family":"Qu","sequence":"first","affiliation":[{"name":"State Key Lab of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"},{"name":"Immunology Department, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Lab of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"},{"name":"Immunology Department, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Lab of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Lab of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"},{"name":"Immunology Department, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Zeng","sequence":"additional","affiliation":[{"name":"National Cancer Registration Office, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021 Beijing, P. 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